Calcitonin gene-related peptide (CGRP)

Hua Zhang and A. S. Verkman. Aquaporin-1 Tunes Pain Perception by Interaction with Nav1.8 Na+ Channels in Dorsal Root Ganglion Neurons. February 19, 2010 The Journal of Biological Chemistry, 285, 5896-5906.

...chicken anti-calcitonin gene-related peptide (CGRP; 1:500, Neuromics, Edina, MN)...

Yong-Jing Gao, Ling Zhang, Omar Abdel Samad, Marc R. Suter, Kawasaki Yasuhiko, Zhen-Zhong Xu, Jong-Yeon Park, Anne-Li Lind, Qiufu Ma, and Ru-Rong Ji. JNK-Induced MCP-1 Production in Spinal Cord Astrocytes Contributes to Central Sensitization and Neuropathic Pain. The Journal of Neuroscience, April 1, 2009, 29(13):4096-4108; doi:10.1523/JNEUROSCI.3623-08.2009.

...calcitonin gene-related peptide (CGRP) antibody (chicken, 1:500; Neuromics)... 

Marcie J. Abbey, Vinit V. Patil, Carrie V. Vause, Paul L. Durham. Repression of calcitonin gene-related peptide expression in trigeminal neurons by a Theobroma cacao extract. Journal of Ethnopharmacology 115 (2008) 238–248.

...Dissociated trigeminal ganglion cells were plated directly on PDL (MW 30,000–70,000; Sigma)-coated 13-mm plastic coverslips equivalent to one ganglion per three coverslips in L15 complete medium. All cells were incubated for 48 h at 37^oC then rinsed briefly with phosphate buffered saline (PBS) and treated with 4% paraformaldehyde for 30 min at room temperature and with 0.2% Triton X-100 in PBS for an additional 15 min to fix and permeabilize the cells. Cultures were incubated overnight at 4^oC with chicken anti-CGRP antibodies (1:500 in PBS; Neuromics, Edina, MN) or rabbit anti-VR1 N-Terminus (TRPV1) antibodies (1:1000 in PBS; Neuromics). Immunoreactive proteins were detected following incubation with Rhodamine Red X-conjugated donkey anti-chicken or FITC-conjugated donkey anti-rabbit IgG polyclonal antibodies (1:100 in PBS, Jackson Immuno-Research Laboratories, West Grove, PA) for 1 h at room temperature. Prior to viewing, cells were mounted using Vectashield mounting media (Vector Laboratories, Burlingame, CA) containing 4',6 diamidino-2-phenylindole (DAPI) to identify nuclei. Cells were photographed with an Olympus DP70 camera mounted on an Olympus BX41 fluorescent microscope and image collection and analysis performed using Olympus MicroSuite Five imageprocessing software (Center Valley, PA)... 

Dongsheng Wu, Wenlong Huang, Peter M. Richardson, John V. Priestley and Min Liu. TRPC4 IN RAT DORSAL ROOT GANGLION NEURONS IS INCREASED AFTER NERVE INJURY AND IS NECESSARY FOR NEURITE OUTGROWTH. J. Biol. Chem, 10.1074/jbc.M703177200

...Slides were also stained with mouse anti-200-kDa neurofilament (N52 clone, 1:5000, Sigma, Poole, U.K.) and rabbit anti-P2X3 (1:1000, Neuromics) or rabbit anti-CGRP (1:1000) antibodies to visualize distinct DRG subpopulations.