|Catalog Number||Size||Price (USD)||Shopping Cart|
|RA22105||100 ul||$275.00||Buy Now | Add to Cart|
Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.
Image: Section of rat cerebral cortex stained with NF-M (red), which reveals the perikarya of pyramidal neurons and dendrites and axons surrounding them. The green channel shows staining with a monoclonal antibody to the beta-adrendergic receptor kinase 1. Protocol on data-sheet.
Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes by Category
Image: Western blot of whole rat cerebellum homogenate stained with NF-M, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~145kDa corresponds to rodent NF-M. Human, cow and bovine NF-M run a little slower, at about 160kDa. Protocol on data-sheet.