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Antibodies

Contact Information

Neuromics
5325 West 74th St., Suite 8
Edina, MN 55439

Toll free: 866-350-1500
Int'l phone: 952-374-6161
Fax: 612-677-3976

VR1-C (TRPV1-C Terminus) and Mu Opioid

IHC Images from Various Labs

Featured Reagents:

TRPV1 C-term (GP14100)

Mu Opioid Receptor

Related Reagents:

TRPV (Vanilloid); TRPM; TRPA and TRPC Antibodies

Pain and Inflammation Research Antibodies

Transfection Reagents

Primary Neurons and Astrocytes -Primary human, rat and mouse neurons and astrocytes

Our TRPV1 (VR1) C-terminal antibody (GP14100) has been used extensively in IHC applications.Several customers have sent in images of staining in Spinal Cord and DRG (See Below Links).This antibody has proved particularly useful for double labeling studies in DRG. Although several labs reported to us using this TRPV1 (VR1) C-terminal antibody for Western blots, we do not recommend it for this application.

We have received many requests for TRPV1 for staining in skin for studies of neuropathic pain and complex regional pain syndrome. Here're some related images and related links to publications.

Images: Deep dermis in CRPS skin had normal appearing Pacinian corpuscles and nerves contained numerous TRPV1/CGRP-positive thin caliber axons. Single and double labeled images are for the antigens indicated in the upper right corners. Single labeled components are indicated with red or green symbols for the corresponding fluorophore, and double labeled components are indicated by yellow symbols. (A,B) Nerves in the deep dermis (i.e., at the level of Pacinian corpuscles) of control and CRPS palmar skin contained a mix of large and small caliber axons that were only labeled with PGP (curved and straight green arrows, respectively), and numerous small caliber axons that also labeled with CGRP (yellow arrowheads). Pacinian corpuscles in the CRPS skin appeared to be normal, with the Ab fiber ending located at the core (green chevron). (C) As seen in this CRPS forearm section, CGRP was found on small caliber axons (red arrowhead) that lacked NF expression seen on other small caliber fibers (green straight arrow) and large caliber Ab fibers (green curved arrow). (D–F) Separate single labeled images (D,E) and the corresponding merged image (F) indicated that most of thin caliber fibers labeled with CGRP also co-expressed TRPV1 (yellow arrowheads). Scale bar = 50 lm. Pain 120 (2006) 244–266.

 

Image: Typical images taken from sections prepared from wild-type (A,B) and vanilloid type 1 transient receptor potential receptor (TRPV1) knockout (D,E) mouse dorsal root ganglia following incubation of the sections in a mixture with the anti-TRPV1 and anti-IR antibodies followed by incubation of the sections in a mixture of appropriate secondary antibodies. TRPV1 immunopsitivity (A,D) and IR immunopositivity (B,E) are shown in images taken with filters for fluorescein isothyocyanate and tetramethyl rhodamine isothiocyanate, respectively. Note that the anti-TRPV1 antibody did not produce any immunostaining in sections taken from TRPV1 knockout mice (D). Arrows and crosses in A point to neurons with a clearly visible nucleus which are immunopositive only to TRPV1 and to both TRPV1 and IR, respectively. Arrows and crosses in B and E indicate neurons with a clearly visible nucleus that are positive only to the IR or to both IR and TRPV1, respectively. Note that a significant proportion of the neurons is positive to both TRPV1 and IR in both the wild-type and the TRPV1 knockout mice. After background subtraction, there was no additional digital modification of the images. Pie chart (C) shows the relative number of neurons expressing TRPV1 and IR immunoreactivity in wild-type mice. Note that there is a significant proportion of neurons expressing both TRPV1 immunopositivity and IR immunopositivity. T, TRPV1-immunopositive; I, IR-immunopositive; T + I, TRPV1- and IR-immunopositive. Scale bar = 25 m.
J. Comp. Neurol. 2007, 503 (2), 334-347.

Double immunofluorescence labeling of VR1 C-terminus (TRPV1) and Mu Opioid Receptor in the DRG and spinal cord.

Shao-Rui Chen and Hui-Lin Pan. Loss of TRPV1-Expressing Sensory Neurons Reduces Spinal mu-Opioid Receptors But Paradoxically Potentiates Opioid Analgesia. doi:10.1152/jn.01343.2005.

Images: Confocal images showing the effect of RTX on mu opioid receptor- and TRPV1-immunoreactive DRG neurons and afferent terminals in the spinal cord. A: representative confocal images showing mu opioid receptor (green) and TRPV1 (red) immunoreactivities in DRG neurons of one vehicle- and one RTX-treated rat. Scale bar, 40 um. B: confocal images showing mu opioid receptor (green) and TRPV1 (red) immunoreactivities in afferent terminals in the spinal dorsal horn of 1 vehicle- and 1 RTX-treated rat. Scale bar, 80 um. Inset: high-magnification images (scale bar = 5 um) showing co-localization of mu opioid receptor and TRPV1 immunoreactivity in the lamina I. Co-localization of the mu opioid receptor and TRPV1 immunoreactivity is indicated in yellow when 2 images are digitally merged. All images are single confocal optical sections.

IHC Images from Various Labs

  
IHC: DRG and Spinal Cord
Data Courtesy of Dr. JeungWoon Lee, Massachusetts General Hospital.
IHC: VR1 C-term (GP14100) and VR1 N-term (RA10110) dual labeling
Images courtesy of Alex Kalyuzhny, University of Minnesota.