TRPV1 (VR1) |
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VR1 C-Terminus (TRPV1) - mouse specific
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Pain and Inflammation Research Antibodies
Neurotransmission -Neurotransmission Research Antibody Categories
Sonia K Bhangoo, Matthew S Ripsch, David J Buchanan, Richard J Miller and Fletcher A White. Increased chemokine signaling in a model of HIV1-associated peripheral neuropathy. Molecular Pain 2009, 5:48doi:10.1186/1744-8069-5-48.
...For some double-labeling experiments, slides were then incubated in anti-TRPV1 antibody (rabbit polyclonal, 1:1000; Neuromics), followed by incubation in secondary antibodies (donkey conjugated to CY5)...
Sacha A. Malin, Julie A. Christianson, Klaus Bielefeldt, and Brian M. Davis. TPRV1 Expression Defines Functionally Distinct Pelvic Colon Afferents. J. Neurosci., Jan 2009; 29: ; doi:10.1523/JNEUROSCI.3791-08.2009.
...Each section was stained with rabbit anti-TRPV1 (Neuromics)...
Sefika C Mizrak, Bart M Gadella, Hatice Erdost, Aytekin Ozer, Ana MM van Pelt, Federica MF van Dissel-Emiliani. Spermatogonial stem cell sensitivity to capsaicin: An in vitro study. Reproductive Biology and Endocrinology 2008, 6:52 doi:10.1186/1477-7827-6-52. 743 - 752
Anti TRPV1 antibody staining: Bouin's fixed, paraffin embedded 5 um-thick rat testis sections were deparaffinized and boiled in a microwave oven (700 Watt) 3x10 min in sodium citrate buffer (0.1 mM, pH=6) for antigen retrieval. All subsequent incubations were performed for 1 hour at room temperature. The slides were then blocked with 5 % goat serum in 1 % BSA/PBS and incubated with the rabbit anti human - VR1 antibody (Neuromics, Edina, MN, USA; 1:500 in 1% BSA/PBS). Biotinilated goat anti-rabbit secondary antibody (BA-1000, Vector Labs; 1:200 in 1% BSA/PBS) was then applied. The ABC kit was finally used according to the manufacturer's instructions. Antibody reactivity was finally detected by diaminobenzidine staining (DAB, Sigma, St. Louis, MO, USA). Sections were counterstained with hematoxylin, dehydrated, mounted with Pertex and studied. Goat serum was applied on control sections.
SDS-PAGE and Western blotting: Protein lysates from the cell lines Gc-5spg and Gc-6spg and the control glioma cell line (A10-85) were prepared in RIPA buffer (PBS, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS) including 1 mM phenylmethylsulfonylfluoride. Of each sample, 50 μg were separated on a 12% SDS-polyacrylamide gel and blotted onto a polyvinylidene fluoride membrane (Millipore Corp., Bedford, MA, USA). Western blots were blocked using Blotto-A, containing 5% Protifar (Nutricia, Zoetermeer, The Netherlands) in Tris-buffered saline (10 mM Tris; 150 mM NaCl, pH 7.6), including 0.05% Tween-20. Rabbit polyclonal anti-VR1 antibody (Neuromics) was diluted 1:1000 in Blotto-A and incubated for 1 h at room temperature. Blots were washed with Tris-buffered saline with 0.05% Tween-20. After incubation with goat anti-rabbit-HRP (P-0260 Dako Cytomation, 1:5000 inBlotto-A) secondary antibody for 1 h, blots were incubated with the electrochemiluminescence kit (ECL, Amersham Pharmacia Biotech, Little Chalfont, UK)and exposed to an x-ray film (RX-omat, Kodak, Chalone / Saone, France).
Alexander T. Stein, Carmen A. Ufret-Vincenty, Li Hua, Luis F. Santana, and Sharona E. Gordon. Phosphoinositide 3-Kinase Binds to TRPV1 and Mediates NGF-stimulated TRPV1 Trafficking to the Plasma Membrane. Published online Oct 30 2006. doi:10.1085/jgp.200609576 © The Rockefeller Press.
Sharif Naeini R, Witty MF, Seguela P, Bourque CW (2006) An N-terminal variant of Trpv1 channel is required for osmosensory transduction. Nature Neuroscience. (1):93-8.
Aoki Y, Ohtori S, Takahashi K, Ino H, Douya H, Ozawa T, Saito T, and Moriya H (2005). Expression and Co-Expression of VR1, CGRP, and IB4-Binding Glycoprotein in Dorsal Root Ganglion Neurons in Rats: Differences Between the Disc Afferents and the Cutaneous Afferents. Spine 30(13):1496-1500.
Labrakakis C, Tong C, Weissman T, Torsney C and MacDermott A (2003). Localization and function of ATP and GABAA receptors expressed by nociceptors and other postnatal sensory neurons in rat. J Physiol 549(1):131-142.
Liapi A, and Wood JN (2005). Extensive co-localization and heteromultimer formation of the vanilloid receptor-like protein TRPV2 and the capsaicin receptor TRPV1 in the adult rat cerebral cortex. Eur J Neurosci 22(4):825.
Nakatsuka T, Tsuzuki K, Ling JX, Sonobe H and Gu JG (2003). Distinct Roles of P2X Receptors in Modulating Glutamate Release at Different Primary Sensory Synapses in Rat Spinal Cord. J Neurophys 89: 3243-3252.
Rosenbaum T, Awaya M, and Gordon SE (2002). Subunit modification and association in VR1 ion channels. BMC Neurosci 2002, 3:4.
Zahner MR, Li DP, Chen SR, and Pan HL (2003). Cardiac vanilloid receptor 1-expressing afferent nerves and their role in the cardiogenic sympathetic reflex in rats. J Physiol 551(2):515-523.
Zimov S and Yazulla S (2004). Localization of vanilloid receptor 1 (TRPV1/VR1)-like immunoreactivity in goldfish and zebrafish retinas: Restriction to photoreceptor synaptic ribbons. J Neurocytol 33:441–452.
Zwick M, Davis BM, Woodbury CJ, Burkett JH, Koerber HR, Simpson JF, and Albers KM (2002). Glial Cell Line-Derived Neurotrophic Factor is a Survival Factor for Isolectin B4-Positive, but not Vanilloid Receptor 1-Positive, Neurons in the Mouse. J Neurosci 22(10):4057-4065.
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K. Czaja, G.A. Burns, and R.C. Ritter. Capsaicin-induced neuronal death and proliferation of the primary sensory neurons located in the nodose ganglia of adult rats. Neuroscience. 2008 June 23; 154(2): 621–630.
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(TRPV1) - mouse specific
Kenjiro Matsumoto, Emi Kurosawa, Hiroyuki Terui, Takuji Hosoya, Kimihito Tashima, Toshihiko Murayama, John V Priestley, and Syunji Horie Localization of TRPV1 and contractile effect of capsaicin in mouse large intestine: high abundance and sensitivity in rectum and distal colon. Am J Physiol Gastrointest Liver Physiol, Jun 2009; doi:10.1152/ajpgi.90578.2008
...TRPV1 antibody (mouse TRPV1 C-terminus; Neuromics Inc., Minneapolis, MN, USA) were 1:60...different anti-TRPV1 antibodies (1:60,000, Neuromics Inc., and rat TRPV1 C-terminus, 1:1,000...experiments, the antibody (1:60,000; Neuromics Inc.) was pre-incubated with 10 M of...
Rebecca M. Sappington, Tatiana Sidorova, Daniel J. Long, and David J. Calkins. TRPV1: Contribution to Retinal Ganglion Cell Apoptosis and Increased Intracellular Ca2+ with Exposure to Hydrostatic Pressure. Invest. Ophthalmol. Vis. Sci., Feb 2009; 50: 717 - 728.
...we used rabbit anti-mouse TRPV1 IgG (1:500; catalog number RA14113; Neuromics, Edina, MN) against the absolute C terminus of mouse TRPV1 (EDAEVFKDSMAPGEK)...
Ann M. Bode, Yong-Yeon Cho, Duo Zheng, Feng Zhu, Marna E. Ericson, Wei-Ya Ma, Ke Yao, and Zigang Dong. Transient Receptor Potential Type Vanilloid 1 Suppresses Skin Carcinogenesis.
Cancer Res., Feb 2009; 69: 905 - 913.
...dorsal skin samples (100 mum) were processed and immunostained. For the human skin cancer tissue array, we used anti-TRPV1 (Neuromics), anti-EGFR (Cell Signaling), and Alexa Fluor 488 and 647-conjugated secondary antibodies. Mouse skin samples were immunostained...
Rebecca M Sappington, Tatiana Sidorova, Daniel J Long, and David Calkins. TRPV1: Contribution to Retinal Ganglion Cell Apoptosis and Increased Intracellular Ca2+ with Exposure to Hydrostatic Pressure. (Investigative Ophthalmology and Visual Science. )
© 2008 Investigative Ophthalmology and Visual Science DOI: 10.1167/iovs.08-2321.
Katrin Schnizler, Leonid P. Shutov, Michael J. Van Kanegan, Michelle A. Merrill, Blake Nichols, G. Stanley McKnight, Stefan Strack, Johannes W. Hell, and Yuriy M. Usachev. Protein Kinase A Anchoring via AKAP150 Is Essential for TRPV1 Modulation by Forskolin and Prostaglandin E2 in Mouse Sensory Neurons. The Journal of Neuroscience, May 7, 2008, 28(19):4904-4917; doi:10.1523/JNEUROSCI.0233-08.2008.
Jens Hjerling-Leffler, Mona AlQatari, Patrik Ernfors, and Martin Koltzenburg. Emergence of Functional Sensory Subtypes as Defined by Transient Receptor Potential Channel Expression. The Journal of Neuroscience, March 7, 2007 • 27(10):2435–2443 • 2435.
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VR1 C-terminus (TRPV1)-Guinea Pig
Hao Sun, De-Pei Li, Shao-Rui Chen, Walter N. Hittelman and Hui-Lin Pan. Sensing of Blood Pressure Increase by Transient Receptor Potential Vanilloid 1 Receptors on Baroreceptors. doi:10.1124/jpet.109.160473
...guinea pig anti-TRPV1, dilution 1:1000, Neuromics...
Noritaka Imamachi, Goon Ho Park, Hyosang Lee, David J. Anderson, Melvin I. Simon, Allan I. Basbaum, and Sang-Kyou Han TRPV1-expressing primary afferents generate behavioral responses to pruritogens via multiple mechanisms. PNAS, Jul 2009; 106: 11330 - 11335
...rabbit anti-PLC 3 (a gift from the Sternweis Lab at University of Texas Southwestern, Dallas) and 1:400 guinea pig anti-TRPV1 (Neuromics). Secondary antibodies were conjugated to Alexa Fluor 488 or 568 flurochromes (Molecular Probes). Using a Leica TCS-NT confocal...
Djalil Baiou , Peter Santha , Antonio Avelino , Ana Charrua , Timea Bacskai , Klara Matesz , Francisco Cruz , Istvan Nagy. Neurochemical characterization of insulin receptor-expressing primary sensory neurons in wild-type and vanilloid type 1 transient receptor potential receptor knockout mice. J. Comp. Neurol. 2007, 503 (2), 334-347.
T. NAKAGAWA, K. WAKAMATSU, N. ZHANG, S. MAEDA, M. MINAMI, M. SATOH AND S. KANEKO. INTRATHECAL ADMINISTRATION OF ATP PRODUCES LONG-LASTING ALLODYNIA IN RATS: DIFFERENTIAL MECHANISMS IN THE PHASE OF THE INDUCTION AND MAINTENANCE. Neuroscience 147 (2007) 445–455.
Amol M. Patwardhan, Nathaniel A. Jeske, Theodore J. Price, Nikita Gamper, Armen N. Akopian, and Kenneth M. Hargreaves. The cannabinoid WIN 55,212-2 inhibits transient receptor potential vanilloid 1 (TRPV1) and evokes peripheral antihyperalgesia via calcineurin. PNAS 103 (July 2006): 11393-11398.
Ahluwalia J, Rang H, and Nagy I (2002). The putative role of vanilloid receptor-like protein-1 in mediating high threshold noxious heat-sensitivity in rat cultured primary sensory neurons. Eur J Neurosci 16:1483-1489.
Hong S, and Wiley JW (2005). Early painful diabetic neuropathy is associated with differential changes in the expression and function of vanilloid receptor 1. J Biol Chem 280(1):618-27.
Hwang SJ, Burette A, and Valtschanoff JG (2003). VR1-positive primary afferents contact NK1-positive spinoparabrachial neurons. J Comp Neurol 460:255-265.
Liu M, Liu MC, Magoulas C, Priestly JV, Willmott NJ (2003). Versatile Regulation of Cytosolic Ca2+ by Vanilloid Receptor I in Rat Dorsal Root Ganglion Neurons. J Biol Chem 278(7): 5462-5472.
Price T, Louria M, Candelario-Soto D, Dussor G, Jeske N, Patwardhan A, Diogenes A, Trott A, Hargreaves K and Flores C (2005). Treatment of trigeminal ganglion neurons in vitro with NGF, GDNF or BDNF: effects on neuronal survival, neurochemical properties and TRPV1-mediated neuropeptide secretion. BMC Neurosci 6:4.
Wadachi R and Hargreaves KM (2006). Trigeminal Nociceptors Express TLR-4 and CD14: a Mechanism for Pain due to Infection. J Dent Res 85(1):49-53.
Hiroyuki Ichikawa and Tomosada Sugimoto. The co-expression of P2X3 receptor with VR1 and VRL-1 in the rat trigeminal ganglion. doi:10.1016/j.brainres.2003.11.019. Copyright © 2003 Published by Elsevier Science B.V.
TRPV1-C and Skin
Phillip J. Albrecht, Scott Hines, Elon Eisenberg, Dorit Pud, Deborah R. Finlay, M. Kari Connolly, Michel Pare, Gudarz Davar, Frank L. Rice Pathologic alterations of cutaneous innervation and vasculature in affected limbs from patients with complex regional pain syndrome. Pain, 2006 Feb;120(3):244-66. Epub 2006 Jan 19.
Method of detecting skin stimulation effect - Patent 20040191171
...RA14113; Neuromics, Edina, MN)...