Metal Metalloproteinase 9 (MMP-9)
Simon Schäfer, Stephanie Weibel, Ulrike Donat, Qian Zhang, Richard J Aguilar, Nanhai G Chen and Aladar A Szalay. Vaccinia virus-mediated intra-tumoral expression of matrix metalloproteinase 9 enhances oncolysis of PC-3 xenograft tumors. BMC Cancer 2012, 12:366 doi:10.1186/1471-2407-12-366.
Western Blot: PC-3 cells were infected with GLV-1h68 or GLV-1h255 at a multiplicity of infection (MOI) of 1. Supernatants were collected 24 h post infection (p.i.) and sterile-filtred (0.1 μm filter Schleicher & Schuell Dassel, Germany). Cells were washed once with PBS and lysed by addition of SDS sample buffer (130 mM Tris HCl pH 6.8, 4% (w/v) SDS, 20% (v/v) glycerol, 5.3% (v/v) 2-mercaptoethanol, 0.15 mM bromphenol blue). Supernatants were mixed (1:1) with SDS sample buffer. All samples were denatured, separated by SDS-PAGE and transferred to nitrocellulose membranes. MMP-9 was detected using a polyclonal anti-MMP-9 antibody produced in goat (Neuromics, Edina USA, GT15020) and a HRP-conjugated anti-goat antibody (Sigma, Steinheim Germany, A8919). ECL solution (90 mM p-coumaric acid, 250 mM luminol, 1 M Tris–HCl, pH 8.5) was used as a substrate for the HRP reaction. β-actin was detected using an anti-β-actin antibody produced in mouse (Abcam, Cambridge UK, ab6276) and a HRP-conjugated anti-mouse antibody (Abcam, Cambridge UK, ab6728).
Immunostaining: MMP-9 expression was analyzed in PC-3 tumor sections labeled with MMP-9 antibody (Neuromics, Edina USA, GT15020).
Yasuki Yasuda, Yoko Matsumura, Kazuki Kasahara, Noriko Ouji, Shigeki Sugiura, Keiichi Mikasa, and Eiji Kita. Microbial exposure early in life regulates airway inflammation in mice after infection with Streptococcus pneumoniae with enhancement of local resistance. Am J Physiol Lung Cell Mol Physiol, Sep 2009; doi:10.1152/ajplung.00193.2009
...with 10% milk in TBST (10mM Tris, 0.15M NaCl, 0.1% Tween-20), followed by probing with goat anti-mouse MMP-9 antibodies (Ab) (Neuromics Antibodies, Edina, MN, GT15020; 1:5000)...
Zhi-Yuan Zhang, Zhiren Zhang, Uwe Fauser and Hermann J. Schluesener. Improved outcome of EAN, an animal model of GBS, through amelioration of peripheral and central inflammation by minocycline. DOI. 10.1111/j.1582-4934.2008.00333. © 2008 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
... MMP-9 (matrix metallopeptidase-9; 1:500; Neuromics, Edina, MN, USA)...
Stephanie Weibel, Jochen Stritzker, Matthias Eck, Werner Goebel, and Aladar A. Szalay. Colonization of experimental murine breast tumours by Escherichia coli K-12 significantly alters the tumour microenvironment. 10.1111/j.1462-5822.2008.01122.x. Cellular Microbiology. © 2008 Blackwell Publishing Ltd
...goat anti-mouse MMP-9 antibody (Neuromics, Edina, MN)...