Arthur M. Butt, Ilaria Vanzulli, Maria Papanikolaou, Irene Chacon De La Rocha, Virginia  E. Hawkins. Metabotropic Glutamate Receptors Protect Oligodendrocytes from Acute Ischemia in the Mouse Optic Nerve. Neurochem Res (2017). doi:10.1007/s11064-017-2220-1

...cultures were immersion fixed in 1% paraformaldehyde (PFA) containing 15% picric acid for 10 min. Following washes in phosphate buffered saline (PBS), a blocking stage was performed by incubation in 10% normal goat (NGS) in PBS for 1–2 h, then washes in PBS and incubation overnight with primary antibodies in blocking solution containing 0.01% Triton-X-100: rabbit anti-mGluR5, at 1:1000 (Neuromics); rabbit anti-mGluR2/3, at 1:300 (Upstate). Samples were then washed 3 times in PBS and incubated with the appropriate secondary antibodies conjugated with 488 Alexafluor...

Talakad G Lohith, Emily K Osterweil,Masahiro Fujita, Kimberly J Jenko,Mark F Bear and Robert B Innis. Is metabotropic glutamate receptor 5 upregulated in prefrontal cortex in fragile X syndrome? Molecular Autism20134:15 DOI: 10.1186/2040-2392-4-15

... Immunoblotting was performed using 2 μg/mL primary antibody to mGluR5 ([RA16100] Neuromics)...

Ilaria Vanzulli, Arthur M. Butt. mGluR5 protect astrocytes from ischemic damage in postnatal CNS white matter. Cell Calcium. Available online 30 June 2015.

...: rabbit anti-mGluR5, at 1:1000 (Neuromics)...

Dennis M. Maddox; Gayle B. Collin; Akihiro Ikeda; C. Herbert Pratt; Sakae Ikeda; Britt A. Johnson; Ron E. Hurd; Lindsay S. Shopland; Jürgen K. Naggert; Bo Chang; Mark P. Krebs; Patsy M. Nishina. A Mutation in Syne2 Causes Early Retinal Defects in Photoreceptors, Secondary Neurons, and Müller Glia. Investigative Ophthalmology & Visual Science June 2015, Vol.56, 3776-3787. doi:10.1167/iovs.14-16047.

...anti-mGluR6 (Neuromics, Edina, MN, USA)...

Luis Pérez de Sevilla Müller, Allison Sargoy, Laura Fernández-Sánchez, Allen Rodriguez, Janelle Liu, Nicolás Cuenca and Nicholas Brecha. Expression and cellular localization of the voltage-gated calcium channel α2δ3 in the rodent retina. DOI: 10.1002/cne.23751.

...A guinea pig polyclonal antibody to metabotropic glutamate receptor 6 (mGluR6) (cat. #GP13105; Neuromics, Edina, MN, RRID:AB_2341540)...

Talakad G Lohith, Emily K Osterweil, Masahiro Fujita, Kimberly J Jenko, Mark F Bear, Robert B Innis. Is metabotropic glutamate receptor 5 upregulated in prefrontal cortex in fragile X syndrome? Molecular Autism 2013, 4:15 (24 May 2013).

Tissue samples were homogenized using a mortar and pestle in fresh ice-cold, 50 mM Tris– HCl buffer (1:10 w/v) completing 3 × 10 passes with cooling on ice between homogenizations. Homogenates were centrifuged at 20,000g and 4°C for 25 minutes,followed by removal of the supernatant. Pellets were then resuspended in fresh ice-cold 50 mM Tris–HCl buffer and centrifuged again at the same settings. Pellets were resuspended in fresh ice-cold 50 mM Tris–HCl buffer at a protein concentration of approximately 1 mg of protein/mL. Aliquots were stored in a freezer at −80°C until further use. Protein concentrations were determined using the Bradford protein assay (Bio-Rad, Hercules, CA), and absorption was read at 595 nm

Pyroja Sulaiman,Ying Xu,Marie E. Fina,Shanti R. Tummala,Hariharasubramanian Ramakrishnan, Anuradha Dhingra and Noga Vardi. Kir2.4 Surface Expression and Basal Current Are Affected by Heterotrimeric G-Proteins. doi: 10.1074/jbc.M112.412791. March 8, 2013 The Journal of Biological Chemistry, 288, 7420-7429.

...guinea pig anti-mGluR6 (Neuromics Inc.)...

Susanne tom Dieck,Dana Specht,Nicola Strenzke,Yamato Hida,Vidhyasankar Krishnamoorthy,Karl-Friedrich Schmidt,Eiji Inoue,Hiroyoshi Ishizaki,Miki Tanaka-Okamoto,Jun Miyoshi,Akari Hagiwara,Johann H. Brandstätter,Siegrid Löwel,Tim Gollisch,Toshihisa Ohtsuka,and Tobias Moser. Deletion of the Presynaptic Scaffold CAST Reduces Active Zone Size in Rod Photoreceptors and Impairs Visual Processing. The Journal of Neuroscience, 29 August 2012, 32(35):12192-12203; doi:10.1523/JNEUROSCI.0752-12.2012

... rabbit anti-mGluR6 (1:100 for ICC; Neuromics)...

Hoon Shim, Chih-Ting Wang, Yen-Lin Chen, Viet Q. Chau, Kevin G. Fu, Jianqi Yang, A. Rory McQuiston, Rory A. Fisher, and Ching-Kang Chen. Defective Retinal Depolarizing Bipolar Cells (DBCs) in Regulators of G-protein Signaling (RGS) 7 and 11 Double Null Mice. JBC Papers in Press. Published on February 27, 2012 as Manuscript M112.345751. The latest version is at http://www.jbc.org/cgi/doi/10.1074/jbc.M112.345751.

...Animals were sacrificed by CO2 inhalation and the eyeballs were immediately enucleated. After removal of cornea and lens the resulting eyecups were immersionfixed in 4% paraformaldehyde in 1X PBS at room temperature for 15 minutes. This short fixation time ensured good mGluR6 and Gb5 signals at the OPL. After cryoprotection in 30% sucrose in 1X PBS, the eyecups were embedded in TBS (Richard Allan Scientific, Kalamazoo, MI), sectioned at 20μm thickness, and stained...

Allison Lindsay Dorfman, Nicolás Cuenca, Isabel Pinilla, Sylvain Chemtob, and Pierre Lachapelle Immunohistochemical Evidence of Synaptic Retraction, Cytoarchitectural Remodeling, and Cell Death in the Inner Retina of the Rat Model of Oygen-Induced Retinopathy (OIR). Invest. Ophthalmol. Vis. Sci., Mar 2011; 52: 1693 - 1708

....Rabbit anti-mGluR6 25 -27 Neuromics 1:3000 Calbindin D-28K...

Klaus Ruether, Andreas Feigenspan, Judith Pirngruber, Michael Leitges, Wolfgang Baehr, and Olaf Strauss. PKC{alpha} is Essential for the Proper Activation and Termination of the Rod Bipolar Cell Response. © 2010 by The Association for Research in Vision and Ophthalmology, Inc. doi:10.1167/iovs.09-4704

...rabbit anti-mGluR6 (Neuromics, Edina, MN, USA)...

Jagadeesh Kumar Venkatesan, Sivaraman Natarajan, Karin Schwarz, Sabine I. Mayer, Kannan Alpadi, Venkat Giri Magupalli, Ching-Hwa Sung, and Frank Schmitz. Nicotinamide Adenine Dinucleotide-Dependent Binding of the Neuronal Ca2+ Sensor Protein GCAP2 to Photoreceptor Synaptic Ribbons. J. Neurosci., May 2010; 30: 6559 - 6576 ; doi:10.1523/JNEUROSCI.3701-09.2010.

...polyclonal antibody against mGluR6 (Neuromics/Acris Antibodies; RA13105; 1:500)...

Anna Francesconi, Ranju Kumari, R. Suzanne Zukin, and Dominick P. Purpura. Regulation of Group I mGluR Trafficking and Signaling by the Caveolar/Lipid Raft Pathway. J Neurosci. 2009 March 18; 29(11): 3590–3602.

...rabbit anti-mGluR5 (Neuromics, Edina, MN; Lab Vision, Fremont, CA; Upstate/Millipore)...

Jakob S. Satz, Alisdair R. Philp, Huy Nguyen, Hajime Kusano, Jane Lee, Rolf Turk, Megan J. Riker, Jasmine Hernández, Robert M. Weiss, Michael G. Anderson, Robert F. Mullins, Steven A. Moore, Edwin M. Stone, and Kevin P. Campbell. Visual Impairment in the Absence of Dystroglycan. J. Neurosci., Oct 2009; 29: 13136 - 13146 ; doi:10.1523/JNEUROSCI.0474-09.2009.

 ....anti-mGluR6 (Neuromics)...

Lasani S. Wijetunge, Sally M. Till, Thomas H. Gillingwater, Cali A. Ingham, and Peter C. Kind. mGluR5 Regulates Glutamate-Dependent Development of the Mouse Somatosensory Cortex. The Journal of Neuroscience, December 3, 2008, 28(49):13028-13037; doi:10.1523/JNEUROSCI.2600-08.2008.

...Western blotting was performed as mentioned above and membranes were probed with antibodies against mGluR5 (1:4000, Neuromics)...

Dana Specht, Shu-Biao Wu, Paul Turner, Peter Dearden, Frank Koentgen, Uwe Wolfrum, Marion Maw, Johann Helmut Brandstätter, and Susanne tom Dieck. Effects of Presynaptic Mutations on a Postsynaptic Cacna1s Calcium Channel Colocalized with mGluR6 at Mouse Photoreceptor Ribbon Synapses. Investigative Ophthalmology and Visual Science. DOI:  10.1167/iovs.08-2758

...rb anti-mGluR6 (1:100 ICC; Neuromics, Edina, MN)...

Raymond D. Lund, Shaomei Wang, Bin Lu, Sergej Girman, Toby Holmes, Yves Sauvé, Darin J. Messina, Ian R. Harris, Anthony J. Kihm, Alexander M. Harmon, Feng-Yi Chin, Anna Gosiewska, Sanjay K. Mistry. Cells Isolated from Umbilical Cord  Tissue Rescue Photoreceptors and Visual Functions in a Rodent Model of Retinal Disease. Stem Cells Vol. 25 No. 3 March 2007, pp. 602 -611 doi:10.1634/stemcells.2006-0308; www.StemCells.com
© 2007 AlphaMed Press

....mGluR6 (1:2000, Neuromics)...

Ronald G. Gregg, Maarten Kamermans, Jan Klooster, Peter D. Lukasiewicz, Neal S. Peachey, Kirstan A. Vessey and Maureen A. McCall. Nyctalopin Expression in Retinal Bipolar Cells Restores Visual Function in a Mouse Model of Complete X-Linked Congenital Stationary Night Blindness.  J Neurophysiol 98: 3023-3033, 2007. First published September 19, 2007; doi:10.1152/jn.00608.2007

...anti-mGluR6 (Neuromics, Edina, MN)...

Anjali Rao,  Rebecca Dallman,  Scott Henderson,  and Ching-Kang Chen. Gβ5 Is Required for Normal Light Responses and Morphology of Retinal ON-Bipolar Cells. doi:10.1523/JNEUROSCI.4934-07.2007

...1:100 mGluR6 Neuromics (Northfield, MN)... 


...rabbit polyclonal antibody raised against mGluR5 (Neuromics, dilution 1:300)...