November 2016 Newsletter

We are always delighted to receive customer data. It enables us, when authorized, to share with other researchers to forward their work and broaden product capabilities. For example, it was our customers that discovered that i-Fect could be used to deliver siRNA in-vivo.

This month we are offering a 50 USD reward for any data you share with us. This reward will be in the form of Amazon or iTunes gift cards. We will also select the best of the best and the winner will receive a 200 USD gift card.

Neurons

Transfection of functional HeV glycoproteins and infection with HeV pseudotyped virions.In order to establish the feasibility of carrying out the proposed experiments in primary neurons, we show (figure ) that our assays are amenable to use in primary neurons.  In the experiment, Combined Hippocampus, Cortex, and Ventricular -E18 (Neuromics) were plated, and at 3 days were transfected with plasmids encoding HeV G/F as well as YFP.  On the following day, these cells were infected with HeV or VSV pseudotyped viruses bearing RFP.  In the figure, (A) the phase contrast photos show the differentiated neurons; (B) upon excitation for RFP, the red fluorescence indicates neurons infected by HeV pseudotyped virions; (C) upon excitation for YFP, and the green fluorescence shows the efficiency of transfection in neurons.  This experiment indicates that the proposed experiments can be carried out in primary neurons, which are transfectable and infectable in our systems, and thus supports all the proposed aims. Data Courtesy of Dr. Matteo Porotto, Weill Cornell Medical College. Larger Image 

Save on Primary Human Cells

 Human Microglia

Human Microglia in Culture
Need hard to find human cells? Please contact me directly pshuster@neuromics.com or 612-801-1007. We can together determine your needs and desired outcomes and provide a statement of work with pricing, project milestones and delivery.

We wish you a productive November filled with new and exciting discoveries.

Pete Shuster-Owner and CEO, pshuster@neuromics.com or 612-801-1007

Rose Ludescher-Manager of Customer Satisfaction---rose@neuromics.com or 866-350-1500