Elisabeth Dum , Sabine Fürtinger, Elisabeth Gasser, Anneliese Bukovac, Meinrad Drexel, Ramon Tasan, Günther Sperk. Effective G-protein coupling of Y2 receptors along axonal fiber tracts and its relevance for epilepsy. Neuropeptides. Available online 26 October 2016. http://dx.doi.org/10.1016/j.npep.2016.10.005

... The brains were perfused with 4% paraformaldehyde in phosphate buffered saline (PBS) and 30 μm sections were cut. For immunohistochemistry, we used Y2 receptor antibody (1:1000, RA14112, Neuromics, Edina, Minnesota, USA through Eubio, Vienna, Austria). ... 


Angela M Sparrow, Emily G Lowery-Gionta, Kristen E Pleil, Chia Li, Gretchen M Sprow, Benjamin R Cox, Jennifer A Rinker, Ana M Jijon, José Peňa, Montserrat Navarro, Thomas L Kash and Todd E Thiele. Central Neuropeptide Y Modulates Binge-Like Ethanol Drinking in C57BL/6J Mice via Y1 and Y2 Receptors. Neuropsychopharmacology , (4 January 2012) | doi:10.1038/npp.2011.327

...The whole brain was sliced into 40 μm sections using a Leica VT 1000S vibratome (Leica Microsystems, Nussloch, Germany) and stored in a cryopreserve solution until IR analysis. The sections were cut into three sets and processed with antibodies raised against NPY (Phoenix Pharmaceuticals; 1 : 1000; Thiele et al, 2000), Y1R (Antibody 96106 raised against NPY Y1R was provided by CURE/Digestive Disease Research Center, Antibody/RIA Core, NIH grant DK41301 (Los Angeles, CA); 1 : 25 000; Lyons and Thiele, 2010), or Y2R (Neuromics, Edina, MN; 1 : 4000). Tissues from all experimental groups were run through each assay at the same time for each antibody tested...

Andres Acosta1, Maria D. Hurtado1, Oleg Gorbatyuk, Michael La Sala, David Duncan, George Aslanidi, Martha Campbell-Thompson, Lei Zhang, Herbert Herzog, Antonis Voutetakis, Bruce J. Baum, Sergei Zolotukhin. Salivary PYY: A Putative Bypass to Satiety. PLoS ONE 6(10): e26137. doi:10.1371/journal.pone.0026137. Received: July 22, 2011; Accepted: September 20, 2011; Published: October 10, 2011

...Rabbit anti-Y2R (Dilution: 1:3000) using TSA...

Davor Stanić, Jan Mulder, Masahiko Watanabe, Tomas Hökfelt. Characterization of NPY Y2 receptor protein expression in the mouse brain. II. Coexistence with NPY, the Y1 receptor, and other neurotransmitter-related molecules. DOI: 10.1002/cne.22608. The Journal of Comparative Neurology. Volume 519, Issue 7, pages 1219–1257, 1 May 2011. This references use of NPY Y2 antibody for western blot analysis using mouse hippocampal lysates where a band is seen at 44kDa.

Robina Matyal, Feroze Mahmood, Michael Robich, Hiliary Glazera, Kamal Khabbaza, Philip Hessa, Cesario Bianchia, Robert Hagberga, Shu-Xu Hua, and Frank W. Sellkea. Chronic type II diabetes mellitus leads to changes in neuropeptide Y receptor expression and distribution in human myocardial tissue. European Journal of Pharmacology. Volume 665, Issues 1-3, 31 August 2011, Pages 19-28.

...anti-Y2 from Neuromics (Edina, MN)...

Matthew Klompusa, Winnie Hoa, Keith A. Sharkeya and Derek M. McKaya. Antisecretory effects of neuropeptide Y in the mouse colon are region-specific and are lost in DSS-induced colitis.  Regulatory Peptides Volume 165, Issues 2-3, 10 December 2010, Pages 138-145.

rabbit anti-mouse Y1R antibody (1:1,000; Neuromics, cat#RA24506, Edina, MN, USA) for 48h at 4°C. Following

Galina Burdyga, Andrea Varro, Rod Dimaline, David G. Thompson, and Graham J. Dockray Expression of cannabinoid CB1 receptors by vagal afferent neurons: kinetics and role in influencing neurochemical phenotype. Am J Physiol Gastrointest Liver Physiol, Jul 2010; 299: G63 - G69

...rabbit polyclonal Y2R antibody (1:200; Neuromics Antibodies, Edina, MN)...  

Lixin Wang, Guillaume Gourcerol, Pu-Qing Yuan, S. Vincent Wu, Mulugeta Million, Muriel Larauche, and Yvette Taché. Peripheral peptide YY inhibits propulsive colonic motor function through Y2 receptor in conscious mice. Am J Physiol Gastrointest Liver Physiol (November 5, 2009). doi:10.1152/ajpgi.00349.2009. 

 ...Note: Excellent IHC staining of myenteric plexus and submucosal (mouse distal colon) tissue-Free floating submucosal and LMMP whole mounts of both proximal and distal colon from 3 naïve mice were treated in 10% normal goat serum each for 30 min, and followed by incubation with polyclonal rabbit anti-Y2 serum diluted at 1:1,000 (Neuromics, Inc., Edina, MN)...

Galina Burdyga, Guillaume de Lartigue, Helen E. Raybould, Richard Morris, Rod Dimaline, Andrea Varro, David G. Thompson, and Graham J. Dockray. Cholecystokinin Regulates Expression of Y2 Receptors in Vagal Afferent Neurons Serving the Stomach. The Journal of Neuroscience, November 5, 2008, 28(45):11583-11592; doi:10.1523/JNEUROSCI.2493-08.2008.

...For detection of Y2R in rat tissue we used a rabbit polyclonal Y2R antibody (1:200; Neuromics Antibodies)...

Brumovsky P, Stanic D, Shuster S, Herzog H, Villar M, Hokfelt T. (2005) Neuropeptide Y2 receptor protein is present in peptidergic and nonpeptidergic primary sensory neurons of the mouse. J Comp Neurol. 489(3):328-48.

 
...Full characterization of the NPY Y2 antibody in mouse DRG and spinal cord...
 
 
... (GP14107, raised in guinea pig; Edina,  Minnesota)...
 
 
 
...We tested this hypothesis by performing immunohistochemistry that utilized an antibody to the Y2 receptor (Neuromics, Edina, MN)...