Methyl-CpG Binding Protein 2 (MeCP2) is a nuclear protein that is found to bind to symmetrical methylated CpG dinucleotide sequences. It is a transcriptional modulator that can alter gene expression epigenetically via binding to methylated DNA. It is involved not only in transcriptional silencing, but also in transcriptional activation, chromatin remodeling, and RNA splicing. Mecp2is a X-chromosome-linked gene, highly expressed in neurons. The mutations of Mecp2 are linked to Rett syndrome (RTT), which is a neurodevelopmental, autistic disorder that affects mainly females. Studies show that even the loss of a specific phosphorylation site of MeCP2 (e.g., S80, S421, and S424) disturbs normal maturation of the mammalian brain.
Image: Mouse brain section, which was cut at 45μM on a vibratome after perfusion with 4% parformaldehyde and overnight fixation, was stained with monoclonal antibody at 1: 1,000: MO22150. Phospho-MeCP2 at S421 was mainly associated with methylated DNA in nucleus of activated neuron cells (red). Blue shows DAPI staining of nuclear DNA.