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Image: E18 hippocampal neurons stained with MAPT (red) and Doublecortin (green). The two proteins overlap in the proximal dendrites, but doublecortin is more abundant in the growth cones and periphery. As a result, the periphery appears green while the more proximal regions of the cells are yellow. The single longer process of this cell, presumably an axon, has a low doublecortin content and so appears red. Blue staining is the nuclear DNA. Protocol on datasheet.. Courtesy of Dr. Gerry Shaw, UFL

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Combined Hippocampus, Cortex, and Ventricular 20X Image Courtesy of Dr. Lidia Garner, University of Tennessee HSC. Link to Larger Image.

 "I got 10 million cells total after extraction from the tissue.  At Day 4 they all  developed long axons. Thank you so much for the replacement." 

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Primary rat neurons: Transfection of functional HeV glycoproteins and infection with HeV pseudotyped virions.In order to establish the feasibility of carrying out the proposed experiments in primary neurons, we show (figure ) that our assays are amenable to use in primary neurons.  In the experiment, Combined Hippocampus, Cortex, and Ventricular -E18 (Neuromics) were plated, and at 3 days were transfected with plasmids encoding HeV G/F as well as YFP.  On the following day, these cells were infected with HeV or VSV pseudotyped viruses bearing RFP.  In the figure, (A) the phase contrast photos show the differentiated neurons; (B) upon excitation for RFP, the red fluorescence indicates neurons infected by HeV pseudotyped virions; (C) upon excitation for YFP, and the green fluorescence shows the efficiency of transfection in neurons.  This experiment indicates that the proposed experiments can be carried out in primary neurons, which are transfectable and infectable in our systems, and thus supports all the proposed aims. Data Courtesy of Dr. Matteo Porotto, Weill Cornell Medical College. Larger Image 

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Striatal Neuron Cuture. Courtesy of Dr. Henry Guo, Intracellular Therapies, Inc.

 
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Data Courtesy of Evanna Gleason, Louisiana State University

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Hippocampal Neurons-4 Days in Culture. Courtesy of Emily Mcmains (Gleason Lab), LSU

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Hippocampal Neurons-1 Week in Culture. Courtesy of Emily Mcmains (Gleason Lab), LSU
Hippocampal Neurons Protocol
Courtesy of Emily McMains (Gleason Lab), LSU.
Combined Hippocampal, Cortical, and Ventricular Neurons-Large Image
Courtesy of Dr. Lidia Garner, University of Tennessee HSC
Primary Rat Cortical Neurons Outgrowth Assays
Courtesy of Lauren McGillicuddy and her team at Essen Bioscience

Various Labs

  
Hippocampal Neurons Protocol
Courtesy of Emily McMains (Gleason Lab), LSU.
Combined Hippocampal, Cortical, and Ventricular Neurons-Large Image
Courtesy of Dr. Lidia Garner, University of Tennessee HSC
Primary Rat Cortical Neurons Outgrowth Assays
Courtesy of Lauren McGillicuddy and her team at Essen Bioscience