Raphael Jorand, Sunetra Biswas1, Devin L. Wakefield, Steven J. Tobin, Ottavia Golfetto, Kelsey Hilton, Michelle Ko, Joe W. Ramos, Alexander R. Small, Peiguo Chu, Gagandeep Singh, and Tijana Jovanovic-Talisman. Molecular signatures of mu opioid receptor and somatostatin receptor 2 in pancreatic cancer. Mol. Biol. Cell September 28, 2016.doi: 10.1091/mbc.E16-06-0427
...Antibodies and fluorescent dye conjugation
The following primary antibodies were purchased: anti-MOR (guinea pig polyclonal, Abcam); anti-SSTR2 (rabbit polyclonal, Neuromics); anti-CXCR4 (mouse monoclonal, Neuromics); and anti-cytokeratin 8 & 18 (mouse monoclonal, Cell Marque/Sigma). Purchased purified secondary antibodies include rabbit anti-guinea pig (polyclonal, Abcam); goat anti-rabbit (polyclonal, Abcam); and goat anti-mouse (polyclonal, EMD Millipore). Antibody references are provided in Supplementary methods. Secondary antibodies were labeled with Alexa Fluor 405, Alexa Fluor 22 647 (Life Technologies) or Atto 488 (Sigma) containing an N-hydroxysuccinidimidyl ester (NHS) group for conjugation to proteins. ....