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TRA-1-60 (T cell receptor alpha locus) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.
Image: Human embryonic stem cells stained with TRA-1-60 antibody detected with Fluor 488 anti-mouse IgM secondary antibody.Protocol on Datasheet.
This antibody reacts with the neuraminidase resistant form of the TRA-1-60 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells.
Images: FACS staining of NTERA-2 cells using TRA-1-60 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.
Images: Western blot analysis of TRA-1-60 expression in NTERA-2 cl.D1 whole cell lysate.