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These controls bind to  "house keeping" protein which are generally not altered much in expression as a result of experimental manipulations. This makes them excellent the western blot standards against which the band density of other proteins can be compared.

Image: Western blot of crude extract of the human carcinoma HeLa cell line. Lane 15 was probed with Actin, our monoclonal antibody to all six actin isotypes, giving a strong clean band at an SDS-PAGE molecular weight of 42kDa. lane 16 was probed with our antibody to 14-3-3η (14-3-3 eta), which has an SDS-PAGE molecular weight of 28kDa. Lanes 17 was probed with our monoclonal antibody to vimentin. Protocol on Datasheet.

NameCatalog #TypeSpeciesApplicationsSizePrice
ActinMO22125Mouse IgGB; H; M; P; RIF; WB100 ug$275
GAPDHMO25038Mouse IgMB; Ch; H; M; P; RIHC; WB100 ul$285