Western Blotting Controls
These controls bind to "house keeping" protein which are generally not altered much in expression as a result of experimental manipulations. This makes them excellent the western blot standards against which the band density of other proteins can be compared.
Image: Western blot of crude extract of the human carcinoma HeLa cell line. Lane 15 was probed with Actin, our monoclonal antibody to all six actin isotypes, giving a strong clean band at an SDS-PAGE molecular weight of 42kDa. lane 16 was probed with our antibody to 14-3-3η (14-3-3 eta), which has an SDS-PAGE molecular weight of 28kDa. Lanes 17 was probed with our monoclonal antibody to vimentin. Protocol on Datasheet.
|Actin||MO22125||Mouse IgG||B; H; M; P; R||IF; WB||100 ug||$275|
|GAPDH||MO25038||Mouse IgM||B; Ch; H; M; P; R||IHC; WB||100 ul||$285|