Product Details
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Size | List Price | Price | Cart |
---|---|---|---|
1 ml | $102.00 | Add to Cart |
For purification of Strep-tag® II proteins by gravity flow, low pressure or FPLC. Strep-tag® Buffers and Reagents-Lysis, Elution, Regeneration, Blocking and Washing Buffers. |
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A 36 kDa enzyme and a mutant, both with C-terminal Strep-tag II, were expressed in the cytoplasm of E. coli. 1: Sample of crude lysate after cytosolic expression with pASK-IBA3 2: Sample of flow through during chromatography 3: Sample after the addition of 2.5 mM desthiobiotin M: Molecular size standard (kDa)
A 36 kDa enzyme and a mutant, both with C-terminal Strep-tag II, were expressed in the cytoplasm of E. coli. 1: Sample of crude lysate after cytosolic expression with pASK-IBA3 2: Sample of flow through during chromatography 3: Sample after the addition of 2.5 mM desthiobiotin M: Molecular size standard (kDa)