Product Details
Catalog Number: 2-1311-000
Applications: Targeted Protein Expression
Type: Expression Vector
Species Reactivity: E. Coli
Downloads: Datasheet (pdf)
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5 ug$295.00Add to Cart
DescriptionExpression plasmid. The expression cassette is under transcriptional control of the tetracycline promoter/operator. The expressed recombinant protein will be secreted into the periplasm
Affinity tag

Strep-Tactin® affinity tag (Strep-tag II®) for the purification of recombinant protein. The affinity tag is fused to the N-terminus of the recombinant protein and can be removed by cleavage with Thrombin. The cleavage is enhanced due to a “kinker” site1).

SecretionThe ompA signal sequence directs the expressed protein into the periplasmic space and will be cleaved off during the translocation process.
Bacterial ExpressionExpression is induced upon addition of 200 ug anhydrotetracycline (order no.: 2 0401 001; 2 0401 002) per 1 liter E. coli shaking culture (A550= 0.5).
Expression strainAny E. coli strain. The tet promoter works independently from the genetic background of E. coli.
Resistance Ampicillin.
Form5 ug, dissolved in 10 mM Tris/HCl pH 8.0, 1 mM EDTA; 20 E.
Concentration250 ng/ul.
Storage4 °C for frequent usage, 20 °C for long term storage.
1) Hakes, J.D. & Dixon, J.E. (1992): Anal. Biochem. 202, 293-298.
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