Product Details
Catalog Number: 2-1315-000
Applications: Targeted Protein Expression
Type: Expression Vector
Species Reactivity: E. Coli
Downloads: Datasheet (pdf)
Product Sizes
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5 ug$295.00Add to Cart
DescriptionExpression plasmid. The expression cassette is under transcriptional control of the tetracycline promoter/operator. The expressed recombinant protein will be secreted into the periplasm
Affinity tag

Strep-Tactin® affinity tag (Strep-tag II®) for the purification of recombinant protein. The affinity tag is fused to the N-terminus of the recombinant protein and can be removed by cleavage with TEV protease (tabacco etch virus). TEV protease is a site-specific protease with a seven amino acid recognition site (in pASK-IBA16: ENLYFQG) and cleavage occurs between glutamine (Q) and glycine (G).

Bacterial ExpressionExpression is induced upon addition of 200 ug anhydrotetracycline (order no.: 2 0401 001; 2 0401 002) per 1 liter E. coli shaking culture (A550= 0.5).
Expression strainAny E. coli strain. The tet promoter works independently from the genetic background of E. coli.
Resistance Ampicillin.
Form5 ug, dissolved in 10 mM Tris/HCl pH 8.0, 1 mM EDTA; 20 E.
Concentration250 ng/ul.
Storage4 °C for frequent usage, 20 °C for long term storage.
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