Product Details
Catalog Number: 2-1412-000
Applications: Targeted Protein Expression
Type: Expression Vector
Species Reactivity: E. Coli
Downloads: Datasheet (pdf)
Product Sizes
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5 ug$295.00Add to Cart
DescriptionExpression plasmid. The expression cassette is under transcriptional control of the tetracycline promoter/operator. The expressed  recombinant protein will be secreted into the periplasm

Affinity tag

Strep-Tactin® affinity tag (Strep-tag II®) for the purification of recombinant protein. The affinity tag is fused to the N-terminus of the recombinant protein and can be removed by cleavage with Thrombin. The cleavage is enhanced due to a “kinker” site1) (spacer).

Bacterial Expression Expression is induced upon addition of  200 ug anhydrotetracycline (order no.: 2 0401 001; 2 0401 002) per 1 liter E. coli shaking culture (A550= 0.5).
Expression strainAny E. coli strain. The tet promoter works independently from the genetic background of E. coli.
Resistance
 Ampicillin.
Form5 ug, dissolved in 10 mM Tris/HCl pH 8.0, 1 mM EDTA; 20 ul.
Concentration250 ng/ul.
Storage4 °C for frequent usage, 20 °C for long term storage.

1) Hakes, J.D. & Dixon, J.E. (1992): Anal. Biochem. 202, 293-298.

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