Product Details
Catalog Number: 2-1416-000
Applications: Targeted Protein Expression
Type: Expression Vector
Species Reactivity: E. Coli
Downloads: Datasheet (pdf)
Product Sizes
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5 ug$295.00Add to Cart
Description

Expression plasmid. The expression cassette is under transcriptional control of the tetracycline promoter/operator. The expressed recombinant protein will be localized in the cytoplasm.

Affinity tag

Strep-Tactin® affinity tag (Strep-tag II®) for the purification of recombinant protein. The affinity tag is fused to the N-terminus of the recombinant protein and can be removed by cleavage with TEV protease (tabacco etch virus). TEV protease is a site-specific protease with a seven amino acid recognition site (in pASKIBA17plus: ENLYFQG) and cleavage occurs between glutamine (Q) and glycine (G).

SecretionThe ompA signal sequence directs the expressed protein into the periplasmic space and will be cleaved off during the translocation  process.
Bacterial Expression Expression is induced upon addition of  200 ug anhydrotetracycline (order no.: 2 0401 001; 2 0401 002) per 1 liter E. coli shaking culture (A550= 0.5).
Expression strainAny E. coli strain. The tet promoter works independently from the genetic background of E. coli.
Resistance
 Ampicillin.
Form5 ug, dissolved in 10 mM Tris/HCl pH 8.0, 1 mM EDTA; 20 ul.
Concentration250 ng/ul.
Storage4 °C for frequent usage, 20 °C for long term storage.
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