Product Details
Catalog Number: 2-1913-000
Applications: Targeted Protein Expression
Type: Expression Vector
Species Reactivity: Mammalian
Downloads: Datasheet (pdf)
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5 ug$295.00Add to Cart

Description

Eukaryotic expression vector designed for high-level expression and non-replicative transient expression in most mammalian hosts containing the following elements:
  • Human Cytomeglovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells.
  • Multiple cloning sites.
  • Neomycin resistant gene for selection of stable cell lines.
  • Episomal replication in cell lines that are latently transfected SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7).
  • The expressed recombinant will be expressed in the cytoplasm.
  • Strep-Tactin affinity tag (Strep-Tag II) for the purification protein. The affinity tag is fused to the N-terminus of the recombinant protein and can be removed by cleavage with thrombin. Cleavage is enhanced by  a "kinker" site1
Resistance for Selection in E. Coli
 Ampicillin.
Form5 ug, dissolved in TE Buffer (10 mM Tris/HCl, pH 8.0 1mM EDTA), 20 ml.
Concentration250 ng/ul.
Storage4 °C for frequent usage, 20 °C for long term storage.

1) Hakes, J.D. and Dixon J.E.(1992), Anal. Biochem. 202: 293-298.

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