2-4101-001 has been replaced by 2-5101-001, but the product functionality has not changed
Ready-to-use microplates (12 x 8-well strips) for diagnostic/assay applications, coated with Strep-Tactin® XT, a new Strep-Tactin variant with higher affinity for Strep-tag® II fusion proteins (nM affinity) and especially Twin-Strep-tag® fusion proteins (pM affinity). Fusion proteins are selectively captured from complex mixtures and the biomolecules of interest are presented to interacting partners in a uniform manner which results in reliable and reproducible assays. The solid-phase, multi-well format for convenient assays and high throughput screenings is compatible with standard multichannel pipettes and automated plate washers and plate readers.
Twin-Strep-tag® fusion protein binding capacity (determined with bacterial alkaline phosphatase (97 kDa)) for 200µl incubation volume: 4 pmol Twin-Strep-tag® fusion protein/well.
Elution of the bound fusion proteins is possible with biotin.
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- Nadine Vollack-Hesse, et al. (2021). Recombinant VWF Fragments Improve Bioavailability of Subcutaneous Factor VIII in Hemophilia A Mice. Blood, 137(8), 1072–1081. doi: 10.1182/blood.2020006468
- Juliette Hordeaux. (2019). The GPI-Linked Protein LY6A Drives AAV-PHP.B Transport across the Blood-Brain Barrier. Molecular Therapy, 27(5), 912-921. doi: 10.1016/j.ymthe.2019.02.013
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