Our 3D Human HUVECs Angiogenesis model is constructed using GFP-Tagged human umbilical vein endothelial cells (HUVECs) are co-cultured with RFP-Tagged supporting cells. GFP positive capillary like tubule formation can be monitored in real time under fluorescence microscope throughout the whole process of the experiment.
The 3D model is designed that the testing materials, i.e. compounds, conditioned media, or tissue explants, can be added into the system at any time, ranging from the onset of vasculogenesis to advanced angiogenesis. The resulting effect on tubule formation (tubular length, number of branches, etc.) can be monitored throughout the whole process under an inverted fluorescence microscope.
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