Product Details
Catalog Number: 3D45001-24
Applications: In vivo Like Assays
Type: 3D Cell/Tissue Models
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
24 Well$1,539.00Add to Cart

Our 3D Human HUVECs Angiogenesis model is constructed using GFP-Tagged human umbilical vein endothelial cells (HUVECs) are co-cultured with RFP-Tagged supporting cells. GFP positive capillary like tubule formation can be monitored in real time under fluorescence microscope throughout the whole process of the experiment.

The 3D model is designed that the testing materials, i.e. compounds, conditioned media, or tissue explants, can be added into the system at any time, ranging from the onset of vasculogenesis to advanced angiogenesis. The resulting effect on tubule formation (tubular length, number of branches, etc.) can be monitored throughout the whole process under an inverted fluorescence microscope.

Images
Left: 4x overlay image of HUVECs used in an endothelial  cell capillary tube formation assay. The cells were stained with calcein.Imaging was performed in the brightfield and GFP channels. Right: Co-culture system

Left: 4x overlay image of HUVECs used in an endothelial cell capillary tube formation assay. The cells were stained with calcein.Imaging was performed in the brightfield and GFP channels. Right: Co-culture system

Left: 4x overlay image of HUVECs used in an endothelial  cell capillary tube formation assay. The cells were stained with calcein.Imaging was performed in the brightfield and GFP channels. Right: Co-culture system

Left: 4x overlay image of HUVECs used in an endothelial cell capillary tube formation assay. The cells were stained with calcein.Imaging was performed in the brightfield and GFP channels. Right: Co-culture system

HUVECs growing in inserts

HUVECs growing in inserts

HUVECs growing in inserts

HUVECs growing in inserts