Alpha-glia Expansion Medium is a complete medium designed to promote optimal growth of normal human brain-derived microglia in vitro. It is a sterile, liquid medium which contains:
The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes growth of normal human microglia in vitro.
Alpha-glia Expansion Medium consists of 445 ml of basal medium, 25 ml of fetal bovine serum 25 ml of microglia growth supplement and 5 ml of penicillin/streptomycin solution.