Product Details
Catalog Number: CH23000
Applications: ICC, WB, IHC
Type: Chicken IgY
Dilutions: ICC 1:100-1:200; IHC 1:100-1:200; WB 1:250-1:500
Immunogen: Peptide common to human (P28329) and mouse (Q03059) ChAT
Storage: Store at 4°C in the dark
Shipping: Ambient
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 1103
UniProt: P28329
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$99.00Add to Cart

Murine choline-O-acetyltransferase (EC 2.3.1.6) is a 71,721 dalton protein(640 amino acids) expressed in cholinergic neurons of both the PNS and CNS.  In the CNS, ChAT is expressed in motor neurons and pre-ganglionic autonomic neurons of the spinal cord, a subset of neurons in the neostriatum, and in the basal forebrain. In the PNS, ChAT is expressed in a small subpopulation of sympathetic neurons and in all parasympathetic neurons. ChAT is the enzyme responsible for synthesis of acetylcholine from acetyl-coenzyme A and choline.

Images
A tissue section through an adult mouse brain showing ChAT (red staining) in cholinergic neurons of the caudate-putamen nucleus.

A tissue section through an adult mouse brain showing ChAT (red staining) in cholinergic neurons of the caudate-putamen nucleus.

A tissue section through an adult mouse brain showing ChAT (red staining) in cholinergic neurons of the caudate-putamen nucleus.

A tissue section through an adult mouse brain showing ChAT (red staining) in cholinergic neurons of the caudate-putamen nucleus.