Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using IgY blocking reagent, and HRP-labeled goat anti-chicken antibodies as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.
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