Product Details
Catalog Number: CH23107
Applications: ICC, WB
Type: Chicken IgY
Immunogen: Purified recombinant human full-length IBA1/AIF1 (Uniprot P55008) expressed in E.Coli
Storage: Store at 4°C in the dark
Shipping: Ambient
Format A: Affinity Purified
Species Reactivity: Human, Mouse, Rat
Entrez: 199
UniProt: P55008
Downloads: Datasheet (pdf)
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AIF1, allograft inflammatory factor-1, (also known as Iba1, Ionized calcium binding adaptor molecule 1) is a 17 kDa protein that is a pro-inflammatory molecule that mediates calcium signals. During inflammation, increased expression of AIF1 occurs in microglia, macrophages, T-cells, synoviocytes, and adipocytes. Expression of AIF-1 has been observed in healthy brains and varies throughout the day, gaining the highest expression in the prefrontal and parietal cortices when sleeping. Increased expression of AIF1 has been observed in several diseases including breast cancer, atherosclerosis, and rheumatoid arthritis.

Images
Immunofluorescence

Immunofluorescence analysis of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.

Immunofluorescence

Immunofluorescence analysis of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.

Western Blot

Western blot of rat brain membrane lysate using chicken α-AIF1/IBA1 showing specific immunolabeling of endocgenous AIF1/IBA1 at ~17kDa.

Western Blot

Western blot of rat brain membrane lysate using chicken α-AIF1/IBA1 showing specific immunolabeling of endocgenous AIF1/IBA1 at ~17kDa.