Leukocyte Common Antigen, CD45, is a transmembrane protein tyrosine phosphatase (1, 2). Isoforms of CD45 are generated by differential glycosylation and by alternative splicing of several exons encoding amino terminal domains of the protein (1, 2). The pattern of expression of different CD45 isoforms is tightly regulated during lymphoid development and is modulated upon antigenic exposure (1, 2).
The 2D1 antibody is produced by a hybridoma generated from the fusion of mouse NS1 myeloma cells with splenic B cells from a BALB/c mouse immunized subcutaneously with human peripheral blood mononuclear cells (3). This antibody recognizes CD45 molecules highly expressed on T cells, B cells, and medulary thymocytes (4). The 2D1 antibody stains CD45 isoforms weakly expressed on granulocytes, monocytes, and macrophages (4). CD45 is not expressed on platelets or erythrocytes (4). Accordingly, CD45 has been used as a marker for all nucleated cells in the peripheral blood.
1. Trowbridge, I.S. (1991) J. Biol. Chem. 266:23517.
2. Hemler, M.E. et al. (1993) in Leukocyte Typing V, eds. Schlossman, S.F. et al., Oxford University Press, Oxford, pp. 1617 - 1618.
3. Beverley, P. (1980) in Proc. XI. International Course on Transplant and Clinical Immunology, Excerpta Medica, Amsterdam. pp. 87 - 94.
4. Pizzolo, G. et al. (1980) Cancer 46:2640.
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