Interleukin 6 (IL-6), also known as interferon-beta 2, 26-kDa protein, B cell stimulatory factor-2 (BSF-2), hybridoma/plasmacytoma growth factor, hepatocyte stimulating factor, cytotoxic T cell differentiation factor, and macrophage-granulocyte inducing factor 2A (MGI-2A), is a multi-functional protein that plays important roles in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts, hepatocytes, keratinocytes, astrocytes, vascular endothelial cells, and various tumor cells.
It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass.
Images: Figure 1-Mouse IL-6 stimulates the proliferation of the IL-6-dependent murine plasmacytoma cells, T1126.96.36.199 in a dose-dependent manner (Nordan, R.P. and M. Potter, 1986, Science 233:566 - 569). The ED 50for this effect is typically 0.02 - 0.06 ng/mL.
Figure 2-To measure the ability of the antibody to neutralize the bioactivity of rmIL-6 on T1188.8.131.52 cells, rmIL-6 was incubated with various concentrations of the antibody for 1 hour at 37° C in a 96 well plate. Following this preincubation period, T1184.108.40.206 cells were added. The assay mixture in a total volume of 100 μL, containing antibody at the concentrations indicated, rmIL-6 at 0.25 ng/mL and cells at 2 x 105 cells/mL, was incubated at 37° C for 72 hours in a humidified CO2 incubator. Resazurin was added during the final 16 - 20 hours of incubation to measure cell growth. The relative fluorescence was then read in a fluorescent plate reader set at Ex. 544/Em. 590. The ND of the antibody is approximately 0.01 - 0.03 μg/mL.
- Product Reviews
- Product Publications
If you've used this product in a publication, let us know. Email firstname.lastname@example.org, with the publication details and you could be eligible for an Amazon gift card.
- Related Products
Name Catalog # Size CXCL8/IL-8 GT15156 100 ug Fractalkine(CX3CL1) GT15024 100 ug Human IL-6 Antibody MO15160 100 ug IL-1 alpha/IL-F1 GT15101 100 ug IL-1 beta (Mouse) GT15102 100 ug IL-1 beta (Rat) GT15105 100 ug IL-17 MO15110 500 ug IL-17E/IL-25 MO15111 500 ug IL-2 MO15069 100 ug IL-2 MO15069 500 ug IL-2 (Rat) GT15155 100 ug IL-4 MO15071 100 ug IL-4 MO15071 500 ug IL-6 (Rat) GT15106 100 ug Macrophage Inflammatory Protein 1alpha (MIP-1a)/CCL3 GT15070 100 ug Macrophage Inflammatory Protein 1alpha (MIP-1a)/CCL3 GT15070 50 ug Macrophage Inflammatory Protein 1beta (MIP-1beta)/CCL4 GT15071 100 ug Porcine IL-6 Antibody GT15264 50 ug TNF alpha GT15113 100 ug