Product Details
Catalog Number: GT15195
Applications: WB
Type: Goat IgG
Dilutions: WB 1:500 - 1:1000
Immunogen: Purified, E. coli-derived recombinant human Livin ?, also known as ML-IAP (amino acids 1 - 280), with a carboxyl-terminal six histidine tag (GenBank accession # AF311388).
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Rat
Entrez: 79444
UniProt: Q96CA5
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$365.00Add to Cart

The protein encoded by this gene is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Two transcript variants encoding different isoforms have been found for this gene. The two isoforms have different antiapoptotic properties, with isoform alpha protecting cells from apoptosis induced by staurosporine and isoform b protecting cells from apoptosis induced by etoposide.

Images

Detection of Livin α and β. 0.5 ng of His tagged recombinant human Livin-α and human Livin-β , and lysates from 0.5 x 106 human SK-Mel-28 cells were resolved by SDS-PAGE, transferred to Immobilon membrane and immunoblotted with 0.5 μg/mL goat anti-Livin. A 60 second exposure to film is shown.

Detection of Livin α and β. 0.5 ng of His tagged recombinant human Livin-α and human Livin-β , and lysates from 0.5 x 106 human SK-Mel-28 cells were resolved by SDS-PAGE, transferred to Immobilon membrane and immunoblotted with 0.5 μg/mL goat anti-Livin. A 60 second exposure to film is shown.