FAS Ligand, also known as CD95L or APT1LG1 , is a member of the TNF receptor family.
The full length Fas-L protein has a cytoplasmic domain, transmembrane (Type II) domain and extracellular domain. The membrane-bound form of Fas-L is proteolytically cleaved to produce soluble Fas-L. There are at least 2 known isoforms of Fas-L produced by alternate splicing.
Fas ligand-receptor interactions play an important role in the regulation of the immune system and the progression of cancer.
Figure 1-The biological activity of rhFas Ligand was measured by its ability to induce apoptosis of Jurkat cells (Cifone M.G. et al., 1994, J. Exp. Med. 180:1547). The ED50 of this effect is typically 0.2 - 0.5 ng/mL.
Figure 2-To measure the ability of the antibody to neutralize the bioactivity of rhFas Ligand, rhFas Ligand was incubated with various concentrations of the antibody for 1 hour at 37° C. Following this preincubation period, Jurkat cells were added. The assay mixture in a total volume of 100 μL/well, containing antibody at the concentrations indicated, rhFas Ligand at 2 ng/mL and Jurkat cells at 5 x 104/mL, was incubated for 2 days at 37° C in a 5% CO2 humidified incubator. 3H-thymidine was added during the final 4 hours of the incubation. The cells were subsequently harvested onto a glass fiber filter and the 3H-thymidine incorporation into DNA was determined. The ND50 of this antibody is typically 2 - 8 ng/mL.
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