Product Details
Catalog Number: GT15230
Applications: WB, IHC, E
Type: Goat IgG
Immunogen: Purified, E. coli-derived, recombinant human ADPN (rhADPN; aa 160 - 349; Accession # Q9NST1).
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human
Entrez: 80339
UniProt: Q9NST1
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$365.00Add to Cart

Human ADPN (Adiponutrin; also PNPLA3 and patatin-like phospholipase domain-containing protein 3) is a 53 kDa member of the PNPLA family of phospholipase A2 enzymes. It is expressed in white adipocytes. Adiponutrin is elevated in response to insulin and appears to act not as a phospholipase, but a molecule involved in anabolic pathways. ADPN is likely to be a type II transmembrane protein that is 481 amino acids (aa) in length. It contains one patatin domain (aa 10 - 179) that is associated with in vitro enzyme activity and a transmembrane segment between aa 42 - 62. There appear to be two splice variants. One shows a four aa deletion of aa 59 - 62 and a second shows an alternate start site at Met379. Over aa 160 - 349, human ADPN shares 61% aa identity with mouse Adiponutrin.

Images
ADPN satining of human adipocytes (dilution:10 µg/mL). Cells were stained using 557 donkey anti-sheep IgG secondary antibody (red) and counterstained with DAPI (blue).

ADPN satining of human adipocytes (dilution:10 µg/mL). Cells were stained using 557 donkey anti-sheep IgG secondary antibody (red) and counterstained with DAPI (blue).

ADPN satining of human adipocytes (dilution:10 µg/mL). Cells were stained using 557 donkey anti-sheep IgG secondary antibody (red) and counterstained with DAPI (blue).

ADPN satining of human adipocytes (dilution:10 µg/mL). Cells were stained using 557 donkey anti-sheep IgG secondary antibody (red) and counterstained with DAPI (blue).

Detection of ADPN by western blot. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0 μg/mL sheep anti-hADPN.

Detection of ADPN by western blot. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0 μg/mL sheep anti-hADPN.