Actin is one of the most abundant and highly conserved proteins of eukaryotes. Mammalian actins are the product of six different genes with differing distribution patterns in cell types and in tissues. The molecular weight of all six proteins is 42kDa, and one or more actins is found in essentially every type of crude cellular and tissue extract. Actin is regarded as a "housekeeping" protein which is generally not altered much in expression as a result of experimental manipulations. So quantitation of the actin band on the western blots is used as a standard against which the band density of other proteins can be compared. Glyceraldehyde 3 phosphate dehydrogenase (GAPDH)(MO25038) is another "housekeeping" protein used in this way. Since our antibody binds all six isotypes very strongly on western blots, it is a very effective blotting standard. It also works in immunocytochemical experiments, binding strongly and cleanly to filopodia, membrane ruffles and stress fibers, all known to be rich in actin
Actin is also a marker for autoimmune diseases of the gut and correlates to intestinal damage. It is also associated with certain forms of hepatitis.
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Name Catalog # Size Actin MO22125 100 ug CD146/MCAM MO30005 50 ug CD31/PECAM-1 GT15190 100 ug GFAP CH22102 100 ul GFAP CH23011 200 ul GFAP MO15052 100 ul GFAP MO22136 100 ul GFAP MO45001 100 ug GFAP RA22101 100 ul Glial Fibrillary Acidic Protein (GFAP) MO22101 100 ul GLUT1 RA25078 100 ul GLUT1 (Glucose Transporter Type I)/SCL2A1 MO47056 100 ul JAM-A GT15249 100 ul JAM-A MO15149 100 ug Nestin MO15012 100 ug Occludin MO15148 100 ug VCAM-1/CD106 GT15251 100 ug VCAM-1/CD106 MO15171 100 ug VCAM1/CD106 MO30006 50 ul Von Willebrand Factor (VWF) RA25102 0.1 ml ZO-1/TJP1 GT30002 100 ul