Product Details
Catalog Number: GT15248
Applications: ICC, WB
Type: Sheep IgG
Dilutions: WB: 1 µg/mL, ICC: 5-15 µg/mL
Immunogen: Human platelet­derived Actin
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$499.00Add to Cart

Actin is one of the most abundant and highly conserved proteins of eukaryotes. Mammalian actins are the product of six different genes with differing distribution patterns in cell types and in tissues. The molecular weight of all six proteins is 42kDa, and one or more actins is found in essentially every type of crude cellular and tissue extract. Actin is regarded as a "housekeeping" protein which is generally not altered much in expression as a result of experimental manipulations. So quantitation of the actin band on the western blots is used as a standard against which the band density of other proteins can be compared. Glyceraldehyde 3 phosphate dehydrogenase (GAPDH)(MO25038) is another "housekeeping" protein used in this way. Since our antibody binds all six isotypes very strongly on western blots, it is a very effective blotting standard. It also works in immunocytochemical experiments, binding strongly and cleanly to filopodia, membrane ruffles and stress fibers, all known to be rich in actin

Actin is also a marker for autoimmune diseases of the gut and correlates to intestinal damage. It is also associated with certain forms of hepatitis.

Images

Actin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity¬purified Polyclonal Antibody (Catalog # GT15428) at 10 µg/mL for 3 hours at room temperature.

Actin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity¬purified Polyclonal Antibody (Catalog # GT15428) at 10 µg/mL for 3 hours at room temperature.