Product Details
Catalog Number: GT15251
Applications: WB, IHC, FC
Type: Goat IgG
Dilutions: Western Blot: 0.25 ug/ml Simple Western: 1 ug/ml Flow Cytometry: 0.25 ug/10^6 Cells Immunohistochemistry: 5-15 ug/ml
Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse VCAM?1/CD106 Phe25-Glu698
Storage: Antibody can also be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2° - 8° C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Species Reactivity: Mouse
Entrez: 22329
UniProt: P29533
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$529.00Add to Cart

VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7.

Images
Immunohistochemistry

Immunohistochemistry: VCAM‑1/CD106 was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (GT15251) at 15 µg/mL overnight at 4 °C.

Immunohistochemistry

Immunohistochemistry: VCAM‑1/CD106 was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (GT15251) at 15 µg/mL overnight at 4 °C.

Flow Cytometry

Flow cytometry: Mouse bone marrow cells were stained with Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody.

Flow Cytometry

Flow cytometry: Mouse bone marrow cells were stained with Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody.

Western Blot

Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody.

Western Blot

Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody.