Product Details
Catalog Number: GT15253
Applications: ICC, WB, IHC
Type: Sheep IgG
Dilutions: Western Blot: 1 µg/mL Immunohistochemistry: 3-15 µg/mL Immunocytochemistry: 1-15 µg/mL
Immunogen: E. coli-derived recombinant human Afadin/AF-6 Asn217-Pro392 Accession # P55196
Storage: Antibody can be stored for 6 months -20° C to -70° C. The antibody can be stored at 2° - 8° C for 1 month. Avoid repeated freeze-thaw cycles.
Shipping: Shipped on Ice Packs
Format A: liquid
Species Reactivity: Human
Entrez: 4301
UniProt: P55196
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$499.00Add to Cart

Afadin, also MLLT-4 (Myeloid/Lymphoid or mixed-lineage Leukemia Translocated to Ch 4) and AF6,  is a 200-210 kDa nectin and F-actin-binding intracellular protein. It is ubiquitously expressed, and serves as an intermediary that links nectin with the actin cytoskeleton in tight and adherens junctions (AJs). Afadin is involved in the nectin-mediated recruitment of catenins to zonule adherens complexes, and in the recruitment of ZO-1 to the apical side of AJs for the purpose of forming tight junctions.

Images
Immunohystochemistry

Afadin/AF-6 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human Afadin/AF-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # GT15253)

Immunohystochemistry

Afadin/AF-6 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human Afadin/AF-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # GT15253)

Immunocytochemistry

Afadin/AF-6 was detected in immersion fixed BEAS2B human lung epithelial cell line using Sheep Anti-Human Afadin/AF-6 Antigen Affinity-purified Polyclonal Antibody

Immunocytochemistry

Afadin/AF-6 was detected in immersion fixed BEAS2B human lung epithelial cell line using Sheep Anti-Human Afadin/AF-6 Antigen Affinity-purified Polyclonal Antibody

Western Blot

Western blot shows lysates of HEK293T human embryonic kidney cell line and SK‑OV‑3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Afadin/AF-6 Antigen Affinity-purified Polyclonal Antibody.

Western Blot

Western blot shows lysates of HEK293T human embryonic kidney cell line and SK‑OV‑3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Afadin/AF-6 Antigen Affinity-purified Polyclonal Antibody.