Product Details
Catalog Number: GT15254
Applications: WB, IHC, FC
Type: Sheep IgG
Dilutions: Immunohistochemistry: 5-15 µg/mL Flow Cytometry: 2.5 µg/10^6 Cells Western Blot: 1 µg/mL
Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD47 Gln19-Pro139 Accession # Q08722
Storage: Antibody can be stored for 6 months -20° C to -70° C. The antibody can be stored at 2° - 8° C for 1 month. Avoid repeated freeze-thaw cycles.
Shipping: Shipped on Ice Packs
Format A: liquid
Species Reactivity: Human
Entrez: 961
UniProt: Q08722
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$509.00Add to Cart

CD47 (also integrin-associated protein/IAP and OA3) is a variably glycosylated, 40‑60 kDa atypical member of the Ig-Superfamily. It is expressed on almost all cell types, including erythrocytes. CD47 binds to TSP-1 and SIRP alpha, and forms a membrane complex with CD36 and  alpha v beta 3.

Images
Immunohistochemistry

CD47 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 µg/mL Sheep Anti-Human CD47 Antigen Affinity-purified Polyclonal Antibody overnight at 4 °C

Immunohistochemistry

CD47 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 µg/mL Sheep Anti-Human CD47 Antigen Affinity-purified Polyclonal Antibody overnight at 4 °C

Flow Cytometry

Human whole blood lymphocytes were stained with Sheep Anti-Human CD47 Antigen Affinity‑purified Polyclonal Antibody

Flow Cytometry

Human whole blood lymphocytes were stained with Sheep Anti-Human CD47 Antigen Affinity‑purified Polyclonal Antibody

Western Blot

Western blot shows lysates of U937 human histiocytic lymphoma cell line and human placenta tissue, not heated to minimize aggregation. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD47 Antigen Affinity-purified Polyclonal Antibody

Western Blot

Western blot shows lysates of U937 human histiocytic lymphoma cell line and human placenta tissue, not heated to minimize aggregation. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD47 Antigen Affinity-purified Polyclonal Antibody