Product Details
Catalog Number: GT15256
Applications: WB, IHC, FC
Type: Goat IgG
Dilutions: Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Flow Cytometry: 0.25 µg/10^6 Cells
Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse B7?1/CD80 Asp37-Lys245 Accession # Q00609
Storage: Antibody can be stored for 6 months -20° C to -70° C. The antibody can be stored at 2° - 8° C for 1 month. Avoid repeated freeze-thaw cycles.
Shipping: Shipped on Ice Packs
Format A: liquid
Species Reactivity: Mouse
Entrez: 941
UniProt: Q00609
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$509.00Add to Cart

Detects mouse B7‑1/CD80 in ELISAs and Western blots. B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses.

Images
Immunohistochemistry

B7‑1/CD80 in Mouse Splenocytes. B7‑1/CD80 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody at 15 µg/mL for 3 hours at room temperature.

Immunohistochemistry

B7‑1/CD80 in Mouse Splenocytes. B7‑1/CD80 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody at 15 µg/mL for 3 hours at room temperature.

Western Blot

Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody

Western Blot

Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody

Flow Cytometry

Mouse splenocytes either treated with 200 ng/mL LPS (filled histogram) or unstimulated (open histogram) were stained with Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody

Flow Cytometry

Mouse splenocytes either treated with 200 ng/mL LPS (filled histogram) or unstimulated (open histogram) were stained with Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody