Product Details
Catalog Number: GT15260
Applications: ICC, WB
Type: Mouse IgG
Dilutions: Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL
Immunogen: E. coli-derived recombinant human Beclin 1 Met12-Gln239
Storage: Antibody can be stored for 6 months -20° C to -70° C. The antibody can be stored at 2° - 8° C for 1 month. Avoid repeated freeze-thaw cycles.
Shipping: Shipped on Ice Packs
Format A: liquid
Species Reactivity: Human, Mouse
Entrez: 596
UniProt: Q14457
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$479.00Add to Cart

Detects human and mouse Beclin 1 in Western blots. Beclin 1 (Bcl-2 interacting coiled-coil protein; also know as ATG6) is a 60 kDa member of the Beclin family of proteins. It is part of a lipid-kinase complex that includes UVRAG and PI3 and induces autophagy; in the presence of Bcl-2, autophagy is repressed. Beclin 1 is widely expressed but abundantly found in mammary epithelium, glia and neurons.

Images
Immunocytochemistry

Beclin 1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse Beclin 1 Antigen Affinity-purified Polyclonal Antibody at 15 µg/mL for 3 hours at room temperature.

Immunocytochemistry

Beclin 1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse Beclin 1 Antigen Affinity-purified Polyclonal Antibody at 15 µg/mL for 3 hours at room temperature.

Western Blot

Western blot shows lysates of T47D human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, CH1 mouse B cell lymphoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Beclin 1 Antigen Affinity‑purified Polyclonal Antibody.

Western Blot

Western blot shows lysates of T47D human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, CH1 mouse B cell lymphoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Beclin 1 Antigen Affinity‑purified Polyclonal Antibody.