Human Umbilical Vein Endothelial Cells

HUVECs were isolated from normal human umbilical vein. Passage 1, cells are shipped frozen or in proliferating culture with a confluency of >90 %*. ENDO-Growth medium containing 5% serum and growth supplements are recommended for culture. Cells have an average additional population doubling levels >20 when cultured.

• Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
• Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
• Cytoplasmic PECAM1 >95% positive by immunofluorescence

HUVECs are negative for HIV-1, HBV, HCV, and mycoplasma.

*cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again. As the cells are shipped live, extreme weather (including extreme cold or heat) can be a factor if we are able to ship or not. 

Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom.

• HUVECS in 3-D Action