hMProTM Mesenchymal Stem Progeitors are derived from human embryonic cell line, H9 (WA09). Cells are >80% CD90, CD105, CD166 and CD73 positive and <5% Oct4 positive. Cells have the capabilties to:
Images: (A) Phase contrast image of hMPro™ mesenchymal progenitor cells in culture. (B) Confocal image of hMPro™ cells stained for the early smooth muscle marker (αSMA;green), F-actin (red) and nuclei (blue). Exposure to 10ng/mL of transforming growth factor beta 1 (TGF-β1) for 12 days induces expression of alpha smooth muscle actin (αSMA) in WA09-derived mesenchymal progenitor cells, suggesting their ability to differentiate along the smooth muscle lineage.
Note: Please include a 30 USD dry ice charge when ordering. Pricing is for US and Canada only. Please contact firstname.lastname@example.org or call 952-374-6161 for international pricing.
Cell Type: mesenchymal progenitor cells Species: human Source: derived from human embryonic cell line, H9 (WA09) Morphology: fibroblastic
Quantity: >1x106 cells per vial (viable post-thaw) Shipping method: dry ice
Storage: liquid nitrogen
Growth Properties: adherent, proliferative Medium: αMEM Medium supplemented with 10% FBS, Pen/Strep and L-glutamine
Phenotyping: Cells are >80% CD90, CD105, CD166 and CD73 positive and <5% Oct4 positive. DNA fingerprint: Results are consistent with the presence of a single cell line with no evidence of culture cross contamination. The loci match the DNA fingerprint pattern for the H9 (NIH designation, WA09) hESC line as published in http://stemcells.nih.gov/research/nihresearch/scunit/.
Pathogen testing: This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV. The H9 cell line has been tested and shown to be negative (performed by GIVF Laboratories). Mesenchymal cells are also tested for sterility and mycoplasma contamination.
Research use only