Product Details
Catalog Number: KF17204
Applications: Apoptosis Detection
Type: Kit
Storage: Store at 2°C-8°C.
Shipping: Refrigerated (Polar Packs)
Species Reactivity: All
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 Tests$561.00Add to Cart
25 Tests$195.00Add to Cart

Polycaspase and Caspase in vitro apoptosis kits make it easy to measure cell death and apoptosis in cultured neurons and many other cell types. Just add the reagent to the media, let it incubate 1- 4 hours, wash the cells and read with a fluorescence plate reader, microscope, or flow cytometer.  Apoptotic cells fluoresce red or green.

Images

Jurkat cells were treated with 1 µM staurosporine to induce caspase 9 activity (top), or treated with a control (bottom). Both populations were incubated with ICT’s green FAM-LEHD-FMK FLICA™ caspase 9. Almost all cells in the induced sample (top) fluoresce green therefore they have activated caspase 9. None of the control cells (bottom) fluoresce green, therefore they do not have activated caspase 9 Courtesy of Dr. Brian Lee, ICT

Jurkat cells were treated with 1 µM staurosporine to induce caspase 9 activity (top), or treated with a control (bottom). Both populations were incubated with ICT’s green FAM-LEHD-FMK FLICA™ caspase 9. Almost all cells in the induced sample (top) fluoresce green therefore they have activated caspase 9. None of the control cells (bottom) fluoresce green, therefore they do not have activated caspase 9 Courtesy of Dr. Brian Lee, ICT