MitoPT™ reagent is based on a fluorescent cationic dye, commonly known as JC-1. Just culture your cells and add MitoPT™ to the media. MitoPT™ easily penetrates cells and healthy mitochondria, where it aggregates and fluoresces red. As the mitochondrial membrane potential collapses, MitoPT™ is diffused throughout the cell. Once dispersed, the reagent assumes a monomeric form and fluoresces green. This can be read with a fluorescence plate reader, flow cytometer, or fluorescence microscope (Fig. 10). MitoPT™ excites at 488nm, and emits at >590nm.
• Are easy -Just add the reagent directly to the cell culture and incubate.