Product Details
Catalog Number: KF17308
Type: Kit
Storage: Store at 2°C-8°C.
Shipping: Refrigerated (Polar Packs)
Downloads: Datasheet (pdf)
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25 Tests$185.00Add to Cart

Cathepsins are usually characterized as members of the lysosomal cysteine protease (active site) family and the cathepsin family name has been synomonous with lysosomal proteolytic enzymes.  In actuality, the cathepsin family also contains members of the serine protease (cathepsin A,G) and aspartic protease (cathepsin D,E) families as well. 

These enzymes exist in their processed form as disulfide-linked heavy and light chain subunits with molecular weights ranging from 20-35 kDa.  Cathepsin C is the noted exception, existing as an oligomeric enzyme with a MW ~200 kDa.  Initially synthesized as inactive zymogens, they are post-translationally processed into their active configurations after passing through the endoplasmic reticulum and subsequent incorporation into the acidic environment of the lysosomes.

Protease activity can be detected within whole living cells using Neuromics’ Magic Red™ substrate-based assay kits.  Designated as the MR-Cathepsin product line, these kits allow researchers to quickly visualize intracellular cathepsin activity within their particular experimental cell line.

Images

Lipopolysaccharide-primed J774A.1 cells were incubated with or without DCA (100 μM, 24 h); then, the cells were stained with Lyso Tracker Green DND-26 (Invitrogen) or Magic RedTM Cathepsin B fluorogenic substrate z-Arg-Arg cresyl violet (Neuromics) for 1 h, followed by Hoechst staining for half an hour. Fluorogenic signals were captured by inverted fluorescence microscope (Leica).

Lipopolysaccharide-primed J774A.1 cells were incubated with or without DCA (100 μM, 24 h); then, the cells were stained with Lyso Tracker Green DND-26 (Invitrogen) or Magic RedTM Cathepsin B fluorogenic substrate z-Arg-Arg cresyl violet (Neuromics) for 1 h, followed by Hoechst staining for half an hour. Fluorogenic signals were captured by inverted fluorescence microscope (Leica).

Detection of intracellular cathepsin K activity in THP-1 cells using (z-LR)2-MR-Cathepsin fluorogenic substrate

Detection of intracellular cathepsin K activity in THP-1 cells using (z-LR)2-MR-Cathepsin fluorogenic substrate