This unique plate coating buffer is for use with antibodies and antigens. The pH and ionic strength of this buffer have been specifically formulated to create an optimal coating environment which maximizes the adsorption efficiency of most antibodies and protein antigens onto polystyrene plate surfaces.
This buffer also stabilizes coated proteins by maintaining their tertiary three-dimensional structure during the adsorption process. By stabilizing the adsorbed protein, antigenic regions are preserved, allowing for greater binding reactivity with the detection molecule, thereby enhancing the specific signal.
By generating a higher specific signal, a lower concentration of coat protein may be needed when this buffer is used, thereby saving valuable reagents. There is a finite quantity of protein that may be coated effectively onto microtiter plates. Use of protein concentrations in excess of the optimal range will result in surface saturation and clumping on the plate surface. This produces lower specific signals, and higher nonspecific signals.
The coating buffer contains an antimicrobial agent to retard bacterial growth during the plate coating process, allowing the procedure to be performed at room temperature. Depending on the activity of the coated protein, plates may be stored at 2°-8°C (or even at room temperature for hardy proteins) for several months, or even years.
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