Plate Coating Buffer-CB1

By generating a higher specific signal, a lower concentration of coat protein may be needed when this buffer is used, thereby saving valuable reagents. There is a finite quantity of protein that may be coated effectively onto microtiter plates.  Use of protein concentrations in excess of the optimal range will result in surface saturation and clumping on the plate surface.  This produces lower specific signals, and higher nonspecific signals.

The coating buffer contains an antimicrobial agent to retard bacterial growth during the plate coating process, allowing the procedure to be performed at room temperature. Depending on the activity of the coated protein, plates may be stored at 2°-8°C (or even at room temperature for hardy proteins) for several months, or even years.