Forget the lysates. Assay for apoptosis via caspase activity in whole, living cells with the FLICA® 660 Polycaspase Assay Kit:
This in vitro caspase assay employs a new far-red fluorescent caspase inhibitor probe, FLICA® 660-VAD-FMK, to label active caspase enzymes in living cells or tissue samples. Image the fluorescent signal using fluorescence microscopy or analyze samples for caspase activity by flow cytometry. The cell permeant FLICA caspase detection probe, 660-VAD-FMK, is comprised of an affinity inhibitor peptide sequence (VAD) and a fluoromethyl ketone (FMK) moiety that enable an irreversible, covalent binding event with active caspase enzymes. This newest FLICA probe is labeled with a far-red fluorescent 660 dye reporter, enabling detection via common fluorescence detection methods.
FLICA™ (Fluorescent-Labeled Inhibitor of Caspases) assays offer a simple yet accurate method to measure individual or pan-caspase activity in whole cells. Two sample protocols are outlined below. Detailed instructions are supplied with the kit.
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