Product Details
Catalog Number: KF17371
Applications: Apoptosis Detection
Storage: Store at 2°C-8°C.
Shipping: Refrigerated (Polar Packs)
Format A: liquid
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
50-100 Tests$295.00Add to Cart

Our Necrosis vs Apoptosis Assay kits simultaneously detects both apoptosis associated cytotoxicity events as well as cell death due to necrosis. Loss of the integrity of the cell membrane, indicative of necrosis or late stage apoptosis, is detected using the vital staining dye, 7-aminoactinomycin D (7-AAD), a red fluorescing live/dead stain. This dye easily penetrates cell membrane-compromised cells, binding tightly to GC rich regions of the DNA. Because 7-AAD alone may not detect cells in the early stages of apoptosis, it is essential to use it in combination with the green-fluorescent FAM-FLICA apoptosis detection reagent. Combining these two different types of fluorescent cell-status-indicator reagents within a single test can reveal a significant percentage of cells that are 7-AAD-negative (membrane intact live cells) and yet FAM-FLICA positive (apoptotic).

Images

(A) Cells were treated with a placebo (B) Cells were treated with 1 µM staurosporine to induce apoptosis. Cells were then dually stained with FAM-FLICA and 7-AAD, and analyzed. FAM-FLICA was analyzed on FL-1 and 7-AAD was analyzed on FL-3. (C) Live, unstained cells do not fluoresce (lower left quadrant). Early apoptotic cells fluoresce green with FAM-FLICA. In the non-induced population (A), only 9.8% of cells were apoptotic compared with 97.1% of the induced population.

(A) Cells were treated with a placebo (B) Cells were treated with 1 µM staurosporine to induce apoptosis. Cells were then dually stained with FAM-FLICA and 7-AAD, and analyzed. FAM-FLICA was analyzed on FL-1 and 7-AAD was analyzed on FL-3. (C) Live, unstained cells do not fluoresce (lower left quadrant). Early apoptotic cells fluoresce green with FAM-FLICA. In the non-induced population (A), only 9.8% of cells were apoptotic compared with 97.1% of the induced population.