Autophagy Assay, Red enables researchers to detect and monitor the in vitro development of autophagy in living cells. Autophagy Probe, Red is a cell-permeant aliphatic molecule that fluoresces brightly when inserted in the lipid membranes of autophagosomes and autolysosomes. Autophagy Probe, Red can be readily detected by flow cytometry (Figure) with optimal excitation at 590 nm and peak emission at 620 nm. Autophagy Probe, Red is for research use only. Not for use in diagnostic procedures.