Product Details
Catalog Number: MO15012
Applications: ICC, IHC, FC
Type: Mouse IgG
Immunogen: human Nestin fragment (amino acid residues 618 - 1618) expressing NSO cells
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Protein G Purified
Format B: liquid
Species Reactivity: Human, Primate
Entrez: 10763
UniProt: P48681
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$265.00Add to Cart

Nestin is a  protein expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.

Images
Nestin staining of human glioblastoma cells.

Nestin staining of human glioblastoma cells.

Nestin staining of human glioblastoma cells.

Nestin staining of human glioblastoma cells.

Nestin staining of human neural progenitor cells grafted into the rat brain, 24 weeks post-op

Nestin staining of human neural progenitor cells grafted into the rat brain, 24 weeks post-op. One is of processes running along the host corpus callosum, one of cells in the hippocampus, weakly counterstained with human nuclear antigen (brown). Nestin concentration was 1:1000, overnight at room temp, visualised using the DAKO vector SG kit. Courtesy of Dr. Rike Zietlow, Cardiff University

Nestin staining of human neural progenitor cells grafted into the rat brain, 24 weeks post-op

Nestin staining of human neural progenitor cells grafted into the rat brain, 24 weeks post-op. One is of processes running along the host corpus callosum, one of cells in the hippocampus, weakly counterstained with human nuclear antigen (brown). Nestin concentration was 1:1000, overnight at room temp, visualised using the DAKO vector SG kit. Courtesy of Dr. Rike Zietlow, Cardiff University

Nestin staining of human week-11 fetal neural progenitor cells. Cells were stained using goat anti-mouse Alexa Fluor 488 secondary antibody (green) (Molecular Probe, A-11001) and counterstained with DAPI (blue).

Nestin staining of human week-11 fetal neural progenitor cells. Cells were stained using goat anti-mouse Alexa Fluor 488 secondary antibody (green) (Molecular Probe, A-11001) and counterstained with DAPI (blue).

Nestin staining of human week-11 fetal neural progenitor cells. Cells were stained using goat anti-mouse Alexa Fluor 488 secondary antibody (green) (Molecular Probe, A-11001) and counterstained with DAPI (blue).

Nestin staining of human week-11 fetal neural progenitor cells. Cells were stained using goat anti-mouse Alexa Fluor 488 secondary antibody (green) (Molecular Probe, A-11001) and counterstained with DAPI (blue).

Immunostaining of  hNP1 Progenitors before and after differentiation. The culture was highly homogenous with neural rosettes.

Immunostaining of hNP1 Progenitors before and after differentiation. The culture was highly homogenous with neural rosettes. Differentiation (2007) DOI: 10.1111/j.1432-0436.2007.00256.x

Immunostaining of  hNP1 Progenitors before and after differentiation. The culture was highly homogenous with neural rosettes.

Immunostaining of hNP1 Progenitors before and after differentiation. The culture was highly homogenous with neural rosettes. Differentiation (2007) DOI: 10.1111/j.1432-0436.2007.00256.x

Accurate detection of intracellular antigens with optimized fixation-permeabilization conditions preserving surface antigens. Flow cytometric detection of TUJ1, MAP2 and nestin antigens in BJ fibroblasts and the neural SH-SY5Y cell line

Accurate detection of intracellular antigens with optimized fixation-permeabilization conditions preserving surface antigens. Flow cytometric detection of TUJ1, MAP2 and nestin antigens in BJ fibroblasts and the neural SH-SY5Y cell line. doi:10.1371/journal.pone.0068519.g003

Accurate detection of intracellular antigens with optimized fixation-permeabilization conditions preserving surface antigens. Flow cytometric detection of TUJ1, MAP2 and nestin antigens in BJ fibroblasts and the neural SH-SY5Y cell line

Accurate detection of intracellular antigens with optimized fixation-permeabilization conditions preserving surface antigens. Flow cytometric detection of TUJ1, MAP2 and nestin antigens in BJ fibroblasts and the neural SH-SY5Y cell line. doi:10.1371/journal.pone.0068519.g003