Product Details
Catalog Number: MO15073
Applications: IHC, FC, NB
Type: Mouse IgG
Immunogen: Hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with human CXCR2- transfected NS0 cells.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Protein G Purified
Format B: Ascites Fluid
Species Reactivity: Human
Entrez: 3579
UniProt: P25025
Product Sizes
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100 ug$325.00Add to Cart

Chemokine receptors are seven-transmembrane domain G-protein coupled receptors that mediate the biological activities of chemokines. Most of these receptors exhibit promiscuous binding properties whereby several different chemokines signal through the same receptor. They are named according to the chemokine subfamily they bind. There are currently six CXC-specific receptors designated CXCR1 to CXCR6, eleven CC-specific receptors designated CCR1 to CCR11, and one C receptor, XCR1. CX3CR1 is the receptor for fractalkine.

Neutralization of Human Cell Surface CXCR2 mediated Bioactivity - See download

The exact concentration of antibody required to neutralize human cell surface CXCR2 mediated bioactivity is dependent on the concentration as well as on the number of CXCR2 receptors present on the cell surface (a function of cell type and culture conditions). The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface CXCR2 mediated rhGROα response on responsive cells at a specific GROα concentration. The ND50 for this lot of anti-human CXCR2 antibody was determined to be approximately 1 - 5 µg/mL in the presence of 5 ng/mL rhGROα in a chemotaxis assay using BaF/3 cells transfected with hCXCR2. The specific conditions are described in the figure legends. The ND50 for the neutralization of myeloperoxidase release from human granulocytes is 0.5 - 1.5 µg/mL in the presence of 1 µg/mL rhGROα.

Images
CXCR2 staining of human lymph nodes. Cells were stained using ABC-HRP + AEC (red) and Haematoxylin (blue) counterstain. It is recommended to employ an avidin-biotin blocking step and quench endogenous peroxidase.

CXCR2 staining of human lymph nodes. Cells were stained using ABC-HRP + AEC (red) and Haematoxylin (blue) counterstain. It is recommended to employ an avidin-biotin blocking step and quench endogenous peroxidase.

CXCR2 staining of human lymph nodes. Cells were stained using ABC-HRP + AEC (red) and Haematoxylin (blue) counterstain. It is recommended to employ an avidin-biotin blocking step and quench endogenous peroxidase.

CXCR2 staining of human lymph nodes. Cells were stained using ABC-HRP + AEC (red) and Haematoxylin (blue) counterstain. It is recommended to employ an avidin-biotin blocking step and quench endogenous peroxidase.