Product Details
Catalog Number: MO15078
Applications: WB, IHC
Type: Mouse IgG
Immunogen: Hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human High Temperature Requirement Protein-2 (rhOmi/HtrA2;aa 134 - 458) with a carboxylterminal six histidine tag (GenBank accession # AF141305).
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Protein G Purified
Format B: liquid
Species Reactivity: Human
Entrez: 27429
UniProt: O43464
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$255.00Add to Cart

The mammalian serine protease Omi (also known as HtrA2), was identified as a 49 kDa serine protease, homologous to the bacterial endoprotease HtrA (high-temperature requirement). It is a homotrimeric serine protease that localizes to the mitochondria and is processed to expose an N-terminal reaper-like motif similar to SMAC/Diablo. Omi/HTRA2 is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition.

It is suspected to play a role in Parkinson's, Alzheimer's, Diabetes and certain forms of cancer.

Images

Detection of HtrA2/Omi with MAB1458.Lysates of human SK-MEL-28, MCF-7, HEK293 and HeLa cells were resolved by SDS-PAGE, transferred to Immobilon-P membrane and immunoblotted with 1.0 g/mL monoclonal anti-HtrA2/Omi, as described in Protocols for Immunoblotting. A 45 second exposure to film is shown

Detection of HtrA2/Omi with MAB1458.Lysates of human SK-MEL-28, MCF-7, HEK293 and HeLa cells were resolved by SDS-PAGE, transferred to Immobilon-P membrane and immunoblotted with 1.0 g/mL monoclonal anti-HtrA2/Omi, as described in Protocols for Immunoblotting. A 45 second exposure to film is shown