Product Details
Catalog Number: MO15103
Applications: WB
Type: Mouse IgG
Dilutions: ICC 1:500 -1:1000; IHC 1:500 -1:1000; WB 1:5,000 - 1:10,000
Immunogen: Hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a phosphopeptide containing the human MBP T98 site. Clone: 399126
Storage: Antibody can also be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2° - 8° C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles
Shipping: Frozen (Polar Packs)
Format A: Protein G Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine
Entrez: 4155
UniProt: P02686
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$255.00Add to Cart

Myelin basic protein is reported to account for about 30 of the proteins in myelin found in the central nervous system. It can induce experimental allergic encephalomyelitis (EAE), a T-lymphocyte mediated disease due to delayed-type hypersensitivity; though each animal species appears to respond to a different fragment of the 170 amino acid polypeptide. Four different isoforms have been identified through cDNA cloning. All four of these variants are identical except for the insertion or deletion of two peptide fragments encoded by exons 2 and 5. Myelin basic protein is reported to be expressed in oligodendrocytes, myelin of white matter in the brain and spinal cord and in peripheral nerves, though it is expressed less abundantly in gray matter.

MBP has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. In vitro, MBP is suitable as a substrate for numerous protein kinases, including the ERK and p38 MAP kinases that phosphorylate MBP at T98.

Note: This antibody detects in vitro-phosphorylated MBP of 21 kDa using Western blot. Based on sequence homology of the immunogen, this antibody should react with phosphorylated MBP from most common vertebrate species, including human, mouse, rat, and bovine.

Images

Detection of phosphorylated MBP. Dephosphorylated MBP purified from bovine brain was treated with increasing amounts of recombinant human active p38α (R&D Systems, Catalog # 1806-P3) for 30 minutes at 30° C. 1μg of the reaction mixtures were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1 μg/ml anti-phospho-MBP (T98)

Detection of phosphorylated MBP. Dephosphorylated MBP purified from bovine brain was treated with increasing amounts of recombinant human active p38α (R&D Systems, Catalog # 1806-P3) for 30 minutes at 30° C. 1μg of the reaction mixtures were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1 μg/ml anti-phospho-MBP (T98)