Product Details
Catalog Number: MO15107
Applications: WB, IHC, E, FC
Type: Mouse IgG
Immunogen: Hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived recombinant human v-akt murine thymoma viral oncogene homolog 1 (rhAkt1; aa 2 - 480; Accession # BC000479).
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Protein G Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 207
UniProt: P31749
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$295.00Add to Cart

Akt1, Akt2, and Akt3 make up a family of serine/threonine kinases, often activated downstream of growth factor receptors and PI 3-Kinase. Each Akt family member contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain.

Main roles are inhibition of apoptosis and promotion of proliferation. AKT has been shown to play a role in such metabolic processes as glucose transport, glycogen synthesis, glycolysis, and protein synthesis. Akt perturbations are thought to play an important role in tumorigenesis.

Images

rhAkt1, rhAkt2, rhAkt3 (all 5 ng; lower panel), and lysates of human MCF-7, MDA-MB-123, and mouse TS1 cells (upper panel) were resolved by SDS-PAGE. Following electrophoresis, recombinant proteins and lysates were transferred to an Immobilon-P membrane and immunoblotted with 0.2 μg/mL anti-pan Akt, as described in Protocols for Immunoblotting. A fifteen second exposure to film is shown.

rhAkt1, rhAkt2, rhAkt3 (all 5 ng; lower panel), and lysates of human MCF-7, MDA-MB-123, and mouse TS1 cells (upper panel) were resolved by SDS-PAGE. Following electrophoresis, recombinant proteins and lysates were transferred to an Immobilon-P membrane and immunoblotted with 0.2 μg/mL anti-pan Akt, as described in Protocols for Immunoblotting. A fifteen second exposure to film is shown.

Detection of Akt family members in human MCF-7 cells (filled histogram) by Ms x h Akt monoclonal antibody. The isotype control staining is shown in open histogram.

Detection of Akt family members in human MCF-7 cells (filled histogram) by Ms x h Akt monoclonal antibody. The isotype control staining is shown in open histogram.

Detection of Akt family members in human MCF-7 cells (filled histogram) by Ms x h Akt monoclonal antibody. The isotype control staining is shown in open histogram.

Detection of Akt family members in human MCF-7 cells (filled histogram) by Ms x h Akt monoclonal antibody. The isotype control staining is shown in open histogram.